Isolation and characterization of human intestinal bacteria capable of transforming the dietary carcinogen 2-amino-1-methyl-6-phenylimidazo[4,5-b]pyridine

Applied and Environmental Microbiology
L VanhaeckeT Van de Wiele

Abstract

2-Amino-1-methyl-6-phenylimidazo[4,5-b]pyridine (PhIP) is a carcinogenic heterocyclic aromatic amine formed in meat products during cooking. Although the formation of hazardous PhIP metabolites by mammalian enzymes has been extensively reported, research on the putative involvement of the human intestinal microbiota in PhIP metabolism remains scarce. In this study, the in vitro conversion of PhIP into its microbial derivate, 7-hydroxy-5-methyl-3-phenyl-6,7,8,9-tetrahydropyrido[3',2':4,5]imidazo[1,2-a]pyrimidin-5-ium chloride (PhIP-M1), by fecal samples from 18 human volunteers was investigated. High-performance liquid chromatography analysis showed that all human fecal samples transformed PhIP but with efficiencies ranging from 1.8 to 96% after 72 h of incubation. Two PhIP-transforming strains, PhIP-M1-a and PhIP-M1-b, were isolated from human feces and identified by fluorescent amplified fragment length polymorphism and pheS sequence analyses as Enterococcus faecium strains. Some strains from culture collections belonging to the species E. durans, E. avium, E. faecium, and Lactobacillus reuteri were also able to perform this transformation. Yeast extract, special peptone, and meat extract supported PhIP transformation by the e...Continue Reading

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Sep 18, 2010·Inflammatory Bowel Diseases·Janelle C Arthur, Christian Jobin
May 23, 2012·Proceedings of the National Academy of Sciences of the United States of America·John H Werren
Jun 29, 2010·Applied and Environmental Microbiology·Hiroyuki KubotaKoji Nomoto
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Apr 28, 2019·International Journal of Molecular Sciences·Alicja M NogackaSonia González
Jul 7, 2017·Journal of Agricultural and Food Chemistry·Falco BeerSabine E Kulling

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