Isolation and Characterization of Hydrocarbon-Degrading Yeast Strains from Petroleum Contaminated Industrial Wastewater

BioMed Research International
Boutheina GargouriSami Sayadi

Abstract

Two yeast strains are enriched and isolated from industrial refinery wastewater. These strains were observed for their ability to utilize several classes of petroleum hydrocarbons substrates, such as n-alkanes and aromatic hydrocarbons as a sole carbon source. Phylogenetic analysis based on the D1/D2 variable domain and the ITS-region sequences indicated that strains HC1 and HC4 were members of the genera Candida and Trichosporon, respectively. The mechanism of hydrocarbon uptaking by yeast, Candida, and Trichosporon has been studied by means of the kinetic analysis of hydrocarbons-degrading yeasts growth and substrate assimilation. Biodegradation capacity and biomass quantity were daily measured during twelve days by gravimetric analysis and gas chromatography coupled with mass spectrometry techniques. Removal of n-alkanes indicated a strong ability of hydrocarbon biodegradation by the isolated yeast strains. These two strains grew on long-chain n-alkane, diesel oil, and crude oil but failed to grow on short-chain n-alkane and aromatic hydrocarbons. Growth measurement attributes of the isolates, using n-hexadecane, diesel oil, and crude oil as substrates, showed that strain HC1 had better degradation for hydrocarbon substrates...Continue Reading

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Citations

Nov 5, 2017·Environmental Monitoring and Assessment·Kamalpreet KaurHarpreet Kaur
Feb 23, 2019·Environmental Science and Pollution Research International·Birintha GanapathyNorahim Ibrahim
Jun 9, 2019·Brazilian Journal of Microbiology : [publication of the Brazilian Society for Microbiology]·Lusiane Malafatti-PiccaDerlene Attili-Angelis
May 4, 2017·World Journal of Microbiology & Biotechnology·Ganesh MahidharaVenkata Ramana Ch
Jun 26, 2020·Scientific Reports·Jossue Ortiz-ÁlvarezCésar Hernández-Rodríguez
Sep 25, 2020·Biotechnology and Applied Biochemistry·D Morales-GuzmánM R Trejo-Hernández
Nov 22, 2020·Applied Microbiology and Biotechnology·Annett MikolaschFrieder Schauer
Oct 30, 2021·Frontiers in Genome Editing·Deeva UthayakumarRebecca S Shapiro

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Datasets Mentioned

BETA
JN088216

Methods Mentioned

BETA
biodegradation assay
PCR

Software Mentioned

MEGA
BLAST
MUSCLE

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