Isolation and characterization of IgE receptors from rat intestinal mucosal mast cells

European Journal of Immunology
M SwieterD Befus

Abstract

High-(Fc epsilon RI) and low-(Fc epsilon RII) affinity IgE receptors were isolated from surface radioiodinated, Nonidet-P40-solubilized rat intestinal mucosal mast cells (IMMC) and compared with those on rat peritoneal mast cells (PMC) and rat basophilic leukemia (RBL) cells. Fc epsilon RII were isolated by affinity chromatography using IgE-Sepharose or by anti-Fc epsilon RII antisera and protein A-Sepharose. The surface-exposed, IgE-binding alpha subunits of Fc epsilon RI [Fc epsilon RI alpha] were isolated by affinity chromatography using IgE and anti-IgE-Sepharose. Fc epsilon RI alpha on IMMC had an apparent molecular mass of 59 kDa, somewhat larger than that of PMC (51 kDa), RBL-2H3 cells (51 kDa) or RBL-CA10.7 cells (46 kDa). Brief (45 s) incubation of IMMC or PMC in glycine-HCl, pH 3, prior to iodination removed much of the surface-bound IgE. This permitted more thorough labeling of the receptors, but had no affect on the estimate of receptor size. Surprisingly and in contrast to acid-treated PMC, upon anti-IgE-Sepharose isolation acid-treated IMMC yielded an intensely radioactive Fc epsilon RI alpha band in the absence of added IgE. Such a finding suggests that IMMC, more so than PMC, may have an intracellular store of I...Continue Reading

References

Jan 1, 1979·International Archives of Allergy and Applied Immunology·R M HelmA Froese
Mar 1, 1988·Digestive Diseases and Sciences·D BefusM Swieter
Nov 1, 1986·European Journal of Immunology·B M ChanA Froese
Jan 1, 1988·International Archives of Allergy and Applied Immunology·T F Huff, D E Justus
Mar 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·A ShimizuP Leder
Jan 1, 1986·Annual Review of Immunology·H MetzgerR Quarto
Jan 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·E T DaytonR L Stevens
Dec 1, 1974·The Journal of Experimental Medicine·A Kulczycki, H Metzger
Mar 1, 1982·Immunology Letters·P A Roth, A Froese
Feb 1, 1983·International Journal for Parasitology·M C LindsayJ F Williams
Apr 1, 1984·Immunology Today·E E Jarrett, D M Haig

Related Concepts

Differentiation Antigens, Hairy Cell Leukemia
Chromatography, Affinity
Structure of Intestinal Gland
Mast Cell
Greater Sac of Peritoneum
Fc Receptor
Leu-11 Antigens

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