Isolation and characterization of iridoviruses from the giant toad Bufo marinus in Venezuela

Diseases of Aquatic Organisms
Z ZupanovicA J Robinson

Abstract

In this communication we describe for the first time the isolation of 7 iridoviruses from the toad Bufo marinus and an unknown species of frog Leptodactylus in Venezuela, South America. The viruses are icosahedral with electron-dense cores, each of which is surrounded by an inner membrane, capsid and a cell-derived envelope. The virus(es) have an average vertex to vertex diameter of 160 nm and replicate in the cytoplasm of a range of cell lines. Within the cytoplasm of infected cells, rarefied areas could be observed; structures lacked cellular organelles and contained complete, empty and developing viruses. Results from antigen-capture enzyme-linked immunosorbent assays (ELISA) with polyclonal antibody raised against epizootic haematopoietic necrosis virus (EHNV) indicated cross-reactivity between these isolates, Bohle iridovirus (BIV) and frog virus 3 (FV3). Comparison of polypeptide and genomic profiles indicated that the Venezuelan viruses shared many polypeptides of equivalent molecular weight with type species FV3. There were, however, differences between the group of Venezuelan viruses and FV3 and BIV. The viruses belongs to the family Iridoviridae and the genus Ranavirus.

Citations

Jan 1, 2010·Journal of Virology·James K JancovichBertram L Jacobs
Sep 15, 2009·Journal of Zoo and Wildlife Medicine : Official Publication of the American Association of Zoo Veterinarians·Elizabeth A DriskellZoltan S Gyimesi
Nov 23, 2010·Journal of Virological Methods·Riikka HolopainenHannele Tapiovaara
Jan 7, 2010·Journal of Fish Diseases·R J WhittingtonM M Dennis
Dec 29, 2005·Developmental and Comparative Immunology·Gregory D ManieroJacques Robert
Feb 1, 2005·Virology·Jacques RobertJennifer Gantress
Dec 22, 1999·Emerging Infectious Diseases·P DaszakR Speare

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