PMID: 6402012Mar 9, 1983Paper

Isolation and characterization of large (0.5 - 1.0 micron) cytoskeleton-free vesicles from human and rabbit erythrocytes

Biochimica Et Biophysica Acta
K S Leonards, S Ohki

Abstract

Large (0.5 - 1.0 micron) cytoskeleton-free vesicles were obtained, by 'budding', from fresh human and rabbit erythrocytes incubated at 45 degrees C and titrated with EDTA and CaCl2. This process occurs without hemolysis. The isolated vesicles maintain their cytoplasmic integrity and normal membrane orientation, and are resistant to hemolysis over the pH range 5.0 - 11.0 and temperature range 4-50 degrees C. The only membrane proteins detected in vesicles from human erythrocytes were band 3 region polypeptides and bands PAS-1, PAS-2 and PAS-3. Vesicles obtained from rabbit erythrocytes were similarly simple. Because of their size and stability these vesicles are amenable to both kinetic and quantitative analysis using the same experimental techniques employed in studies of synthetic lipid membranes. The results obtained in this study indicate that these vesicles are essentially markedly simplified biological cells, and thus may be useful as a biologically relevant model membrane system for examining the molecular interactions which occur within, across and between cell membranes.

References

Jun 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·N ZakaiA Loyter
Jan 1, 1978·Journal of Supramolecular Structure·T L Steck
Sep 21, 1979·Biochimica Et Biophysica Acta·N SaubermanL M Snyder
Oct 15, 1978·The Biochemical Journal·S J QuirkJ A Lucy
Dec 1, 1974·The Journal of Cell Biology·A Elgsaeter, D Branton
Oct 1, 1974·The Journal of Cell Biology·H PeretzA Loyter
Mar 1, 1972·Nature: New Biology·M S Bretscher
Jul 1, 1974·The Journal of Cell Biology·T L Steck
Aug 17, 1973·Science·M S Bretscher
Jan 1, 1973·Journal of Supramolecular Structure·T L Steck, J Yu
Jun 6, 1980·Biochimica Et Biophysica Acta·D L SiegelD Branton
May 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·D E Golan, W Veatch
Jun 16, 1981·Biochimica Et Biophysica Acta·M N Jones, J K Nickson
Feb 1, 1981·The Journal of Cell Biology·V P Patel, G Fairbanks
Dec 7, 1981·Biochimica Et Biophysica Acta·H MüllerH U Lutz
Mar 1, 1980·Experimental Cell Research·S Majumdar, R F Baker
May 23, 1980·Biochimica Et Biophysica Acta·S MajumdarV K Kalra
Nov 4, 1980·Biochimica Et Biophysica Acta·N Sauberman, L M Snyder
Jan 1, 1963·Archives of Biochemistry and Biophysics·J T DODGED J HANAHAN

❮ Previous
Next ❯

Citations

Jan 1, 1985·The Journal of Membrane Biology·S W HuiA Sen
Jul 6, 2000·Archives of Medical Research·J C Jaime-Pérez, D Gómez-Almaguer
Aug 22, 2013·Microscopy Research and Technique·Timothy MooreVitaly Vodyanoy
May 30, 1998·Biochimica Et Biophysica Acta·M Bobrowska-HägerstrandA Iglic
Jan 10, 2006·Transfusion·Tibor J Greenwalt
Nov 6, 1984·Biochemistry·S Ohki, K S Leonards
Jan 31, 2021·International Journal of Molecular Sciences·Michael C LarsonCheryl A Hillery

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.