Isolation and characterization of mesenchymal stromal cells from human degenerated nucleus pulposus: comparison with bone marrow mesenchymal stromal cells from the same subjects

Spine
Juan F BlancoMaria-Consuelo del Cañizo

Abstract

To identify mesenchymal stromal cells (MSC) from degenerate human nucleus pulposus (NP) and compare them with bone marrow (BM) MSC. To test whether MSC obtained from NP and BM from the same subjects share similar biologic characteristics. Recent studies have proposed biologic strategies for the treatment of intervertebral disc degeneration, including cell therapy. Bone marrow (BM) MSC could be an attractive approach to restore disc function, and there is evidence that NP may contain MSC-like cells. Tissue samples were obtained from degenerate lumbar NP and from iliac crest of the same 16 patients with degenerative disc diseases, undergoing discectomy and fusion procedures. MSC isolated from both sources were compared regarding their expansion time, immunophenotype, differentiation ability, and molecular analysis. In all cases, MSC from NP were isolated and expanded. They fulfil nearly all morphological, inmunophenotypical, and differentiation criteria described by the International Society of Cell Therapy for MSC, with the exception that NP-MSC are not able to differentiate into adipocytes. Slight differences were observed with BM-MSC from the same subjects. The NP contains mesenchymal stem cells. These cells were quite similar...Continue Reading

References

Jan 22, 2005·Clinical Orthopaedics and Related Research·Yin-Gang ZhangJun Li
Aug 16, 2005·Blood·Willis H Navarro, Lawrence D Kaplan
Sep 3, 2005·Spine·David Greg AndersonAlexander R Vaccaro
Nov 18, 2005·Biochemical and Biophysical Research Communications·Nobuyuki FujitaToshio Suda
Dec 21, 2005·Blood Reviews·Francesco DazziIrene Roberts
Apr 6, 2006·The Journal of Bone and Joint Surgery. American Volume·Jeffrey N Katz
Apr 6, 2006·The Journal of Bone and Joint Surgery. American Volume·Thijs GrunhagenJill P G Urban
Jul 18, 2006·European Spine Journal : Official Publication of the European Spine Society, the European Spinal Deformity Society, and the European Section of the Cervical Spine Research Society·Victor Y L LeungKenneth M C Cheung
Oct 12, 2007·The New England Journal of Medicine·Frederick R Appelbaum
Nov 17, 2007·Spine·James F GriffithPing Chung Leung
May 13, 2008·Experimental Hematology·Soraya CarrancioM Consuelo Del Cañizo
Sep 13, 2008·Cell Stem Cell·Arnold I Caplan
Dec 17, 2008·Current Stem Cell Research & Therapy·Richard L Haspel, Kenneth B Miller
Feb 5, 2009·Cytotherapy·Reena JethvaEdwin M Horwitz
May 28, 2009·Journal of Cellular Physiology·Anshu RastogiAdam H Hsieh
Jul 3, 2009·Cell Stem Cell·Farshid GuilakChristopher S Chen

❮ Previous
Next ❯

Citations

Oct 29, 2011·European Spine Journal : Official Publication of the European Spine Society, the European Spinal Deformity Society, and the European Section of the Cervical Spine Research Society·Bo HuangYue Zhou
Jul 28, 2011·Transplantation·Lluis OrozcoJavier García-Sancho
May 18, 2013·Transplantation·Lluis OrozcoJavier García-Sancho
May 9, 2012·Advances in Orthopedics·Fadi TaherFederico P Girardi
Feb 1, 2013·Journal of Translational Medicine·Karin BenzWilhelm K Aicher
Dec 12, 2013·BMC Research Notes·Tsung-Han LeeTao-Chen Lee
Oct 10, 2013·Tissue Engineering. Part a·Hai WangCheng-Jie Xiong
Dec 4, 2013·European Spine Journal : Official Publication of the European Spine Society, the European Spinal Deformity Society, and the European Section of the Cervical Spine Research Society·Nevenka Kregar VelikonjaSally Roberts
Apr 30, 2014·European Spine Journal : Official Publication of the European Spine Society, the European Spinal Deformity Society, and the European Section of the Cervical Spine Research Society·Ricardo Rodrigues-PintoJudith A Hoyland
Jul 6, 2014·Advanced Drug Delivery Reviews·Daisuke Sakai, Sibylle Grad
Jan 24, 2013·The Spine Journal : Official Journal of the North American Spine Society·Yong-Can HuangKeith D K Luk
Sep 29, 2011·The Orthopedic Clinics of North America·Daisuke Sakai
Jul 3, 2015·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Twishi GulatiAshish D Diwan
Mar 22, 2016·The Spine Journal : Official Journal of the North American Spine Society·Ching-Hua YehXudong Joshua Li
Mar 18, 2016·European Spine Journal : Official Publication of the European Spine Society, the European Spinal Deformity Society, and the European Section of the Cervical Spine Research Society·Hai WangBo Huang
Mar 29, 2014·Birth Defects Research. Part C, Embryo Today : Reviews·Wilson C W ChanDanny Chan
Jun 13, 2012·Journal of Anatomy·Girish PattappaSibylle Grad
Dec 3, 2014·Advanced Drug Delivery Reviews·Gerd KleinWilhelm K Aicher
Aug 6, 2013·Biotechnology Advances·Joana Silva-CorreiaRui L Reis
Feb 25, 2015·Nature Reviews. Rheumatology·Daisuke Sakai, Gunnar B J Andersson
Apr 13, 2013·The Spine Journal : Official Journal of the North American Spine Society·Olga MizrahiZulma Gazit
Nov 8, 2014·PloS One·Tao-Chen LeeMartin Hsiu-Chu Lin
Feb 19, 2016·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Nam V VoJames D Kang
Mar 2, 2013·Acta Biochimica Et Biophysica Sinica·Miersalijiang YasenFeng Zhang
Dec 21, 2016·Clinical Anatomy : Official Journal of the American Association of Clinical Anatomists & the British Association of Clinical Anatomists·Malik GhannamR Shane Tubbs

❮ Previous
Next ❯

Related Concepts

Related Feeds

CREs: Gene & Cell Therapy

Gene and cell therapy advances have shown promising outcomes for several diseases. The role of cis-regulatory elements (CREs) is crucial in the design of gene therapy vectors. Here is the latest research on CREs in gene and cell therapy.

Allogenic & Autologous Therapies

Allogenic therapies are generated in large batches from unrelated donor tissues such as bone marrow. In contrast, autologous therapies are manufactures as a single lot from the patient being treated. Here is the latest research on allogenic and autologous therapies.