Isolation and characterization of monomeric and dimeric CP47-reaction center photosystem II complexes.

The Journal of Biological Chemistry
D ZhelevaJ Barber

Abstract

Using the detergents n-dodecyl beta-D-maltoside and heptyl thioglycopyranoside, a subcore complex of photosystem II (PSII) has been isolated that contains the chlorophyll-binding protein, CP47, and the reaction center components, D1, D2, and cytochrome b559. We have found, by using sucrose density centrifugation, that the resulting preparation consisted of a mixture of dimeric and monomeric forms of the CP47 reaction center (RC) complex, having molecular masses of 410 +/- 30 and 200 +/- 28 kDa, respectively, as estimated by size exclusion chromatography. The level of the dimer in the preparation is significantly higher than the monomeric form. Both the monomer and dimer contain the proteins CP47, D1, and D2 and the alpha- and beta-subunits of cytochrome b559. Analyses by mass spectrometry and N-terminal sequencing showed that both forms of the CP47-RC complex contain the products of the psbI, psbTc (chloroplast gene), and psbW with molecular masses of 4195.5, 3849.6, and 5927.4 Da, respectively. In contrast to the monomeric form, the CP47-RC dimer contained two extra proteins with low molecular weights, identified as the products of the psbL and psbK genes having molecular masses of 4365.5 and 4292.1, respectively. It was also ...Continue Reading

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Citations

Apr 14, 2011·Photosynthesis Research·Cristina PaglianoJames Barber
Aug 18, 2000·Biochimie·Y Choquet, O Vallon
Aug 17, 1999·Current Opinion in Structural Biology·J Barber, W Kühlbrandt
Mar 31, 1999·Trends in Biochemical Sciences·J BarberB Hankamer
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Jan 12, 2002·Bioelectrochemistry·James Barber
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Dec 10, 1999·Proceedings of the National Academy of Sciences of the United States of America·C BüchelC Dismukes
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Nov 14, 2012·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·M BoehmP J Nixon
Jan 9, 2003·Proceedings of the National Academy of Sciences of the United States of America·Nobuo Kamiya, Jian-Ren Shen
Apr 14, 2007·Proceedings of the National Academy of Sciences of the United States of America·Tatsuya TomoMamoru Mimuro
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