PMID: 3746297Oct 1, 1986Paper

Isolation and characterization of multilayered sheath membrane rich in glucocerebroside from shrimp ventral nerve

Journal of Neurochemistry
N OkamuraT Saida

Abstract

A membrane fraction rich in glucocerebroside was isolated from homogenates of ventral nerves of pink shrimp (Penaeus duorarum) by sucrose gradient centrifugation. The membrane fraction was observed at 0.15 M sucrose and was rich in lipids (lipid/protein ratio approximately 15:1). Electron microscopy showed that the fraction was derived from myelin-like multilayered glial membrane ensheathing axons, which has morphological similarities to myelin. Most of the lipids in shrimp nerve, including glucocerebroside, sphingomyelin, phosphatidylcholine, phosphatidylserine, phosphatidylethanolamine, and ethanolamine-plasmalogen, as well as cholesterol, appeared to be concentrated in this fraction. The fatty acids of these phospholipids were exclusively saturated or monounsaturated with C14-C26 chain lengths. The aldehyde moiety of plasmalogens contained only saturated C14-C18 carbon chains. Like glucocerebrosides, the sphingoid base of sphingomyelin consisted mainly of C14-C16 sphingenines and sphinganines, but they also contained significant amounts of C19 and C20 sphinganines. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis of the proteins in this fraction showed several bands in the 23,000-85,000 Mr range. Radioimmunoassay, h...Continue Reading

References

Dec 23, 1976·The New England Journal of Medicine·S R CohenG M McKhann
Oct 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·N OkamuraY Kishimoto
Dec 1, 1985·Journal of Neurochemistry·N OkamuraY Kishimoto
Mar 1, 1969·Journal of Neurochemistry·Y KishimotoR M Burton
Jul 1, 1980·The Anatomical Record·L A Langford, R E Coggeshall
Jun 24, 1983·Science·K ShimomuraY Kishimoto
Aug 1, 1959·The Journal of Biophysical and Biochemical Cytology·K HAMA
Feb 1, 1961·The Journal of Biophysical and Biochemical Cytology·J H LUFT

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Citations

Jun 11, 2009·Neuron Glia Biology·Betty I Roots
Jan 1, 1990·Annals of the New York Academy of Sciences·T V Waehneldt
Aug 18, 2020·Biochimie·Motohide MurateToshihide Kobayashi
Oct 31, 1987·Biochimica Et Biophysica Acta·H YamaguchiR Gould
Oct 31, 1987·Biochimica Et Biophysica Acta·T TanakaR M Gould
Dec 15, 1986·Chemistry and Physics of Lipids·Y Kishimoto
Jan 1, 1989·Comparative Biochemistry and Physiology. B, Comparative Biochemistry·T V WaehneldtY Kishimoto

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