Isolation and characterization of multipotent human keloid-derived mesenchymal-like stem cells

Stem Cells and Development
Jai-Hee MoonSeungkwon You

Abstract

In this study, we report the isolation and characterization of a population of multipotent keloid-derived mesenchymal-like stem cells (KMLSCs) from keloid scalp tissues. These KMLSCs expressed the typical mesenchymal stem cell marker proteins CD13, CD29, CD44, CD90, fibronectin, and vimentin when they were cultured in serum-containing medium and when subsequent exposure to various differentiation media resulted in their differentiation into adipocytes, osteoblasts, chondrocytes, smooth muscle cells, and angiogenic endothelial cells. When KMLSCs were cultured in neural stem culture conditions (i.e., in the presence of epidermal growth factor and fibroblast growth factor 2 in substrate-free conditions), they produced large numbers of neurospheres containing nestin-, CD133-, and SOX2-positive cells that expressed neural-crest stem cell markers. Subsequent exposure of these cells to different differentiation conditions resulted in cells that expressed neuronal cell-, astrocyte-, oligodendrocyte-, or Schwann cell-specific markers. Our study suggests that KMLSCs may be an alternative adult stem cell resource for regenerative tissue repair and auto-transplantation.

References

Sep 26, 1995·Proceedings of the National Academy of Sciences of the United States of America·G P DimriO Pereira-Smith
Sep 1, 1995·The Journal of Investigative Dermatology·D L du CrosJ R Couchman
Feb 1, 1995·Developmental Dynamics : an Official Publication of the American Association of Anatomists·H E YoungP A Lucas
Jan 1, 1997·The Journal of Clinical Investigation·P J Newman
Apr 2, 1999·Science·M F PittengerD R Marshak
Jun 23, 1999·Proceedings of the National Academy of Sciences of the United States of America·J KishimotoR E Burgeson
Oct 8, 1999·Plastic and Reconstructive Surgery·F B NiessenM Kon
Mar 14, 2000·Cell Death and Differentiation·C C ChipevM Simon
Mar 18, 2000·The Journal of Surgical Research·H XueW Zhang
Apr 20, 2000·The Journal of Investigative Dermatology·P J JensenR M Lavker
Sep 5, 2001·Nature Cell Biology·J G TomaF D Miller
Jan 24, 2002·Journal of the American Society of Nephrology : JASN·Nengjen Remi ShihNorimoto Yanagawa
Mar 5, 2002·The Journal of Investigative Dermatology·Greg SzulgitHumphrey Gardner
Dec 12, 2002·Molecular Biology of the Cell·Patricia A ZukMarc H Hedrick
Jun 12, 2003·Annals of the New York Academy of Sciences·Rupert HandgretingerSelim Kuci
Apr 15, 2004·Medical Hypotheses·Shi Chunmeng, Cheng Tianmin
May 22, 2004·Stem Cells·Joachim OswaldCarsten Werner
Nov 2, 2004·Nature Cell Biology·Karl J L FernandesFreda D Miller
Mar 28, 2006·Journal of Cellular and Molecular Medicine·M HeilW Schaper
May 9, 2006·Experimental Neurology·Karl J L FernandesFreda D Miller
May 11, 2006·Differentiation; Research in Biological Diversity·Byung Sun YoonHyun Soo Yoon
Jun 16, 2006·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Ian A McKenzieFreda D Miller
Oct 21, 2006·Stem Cells·Sang-Moon KangDong-Wook Kim
Nov 14, 2006·The Journal of Clinical Investigation·Jeffrey J RossCatherine M Verfaillie
Jan 8, 2008·Bioscience, Biotechnology, and Biochemistry·Yoonjung KhoYunjaie Choi

❮ Previous
Next ❯

Citations

Aug 19, 2009·Stem Cells and Development·Byung Sun YoonSeungkwon You
Oct 25, 2011·Experimental & Molecular Medicine·Bona KimSeungkwon You
Dec 17, 2008·Annals of Anatomy = Anatomischer Anzeiger : Official Organ of the Anatomische Gesellschaft·Stephan TiedeChristina Zechel
Nov 26, 2010·The British Journal of Surgery·S HindochaA Bayat
Nov 23, 2013·Journal of Cellular Biochemistry·Chui-Yee FongAriff Bongso
Dec 17, 2009·Wound Repair and Regeneration : Official Publication of the Wound Healing Society [and] the European Tissue Repair Society·Barbara ShihArdeshir Bayat
Jan 16, 2015·The Journal of Surgical Research·Mark FairweatherMonica M Bertagnolli
Apr 1, 2016·Journal of Clinical Pathology·Chelsea GrantSwee T Tan
Jan 18, 2014·Stem Cells Translational Medicine·Anna I ArnoMarc G Jeschke
Feb 2, 2017·Acta neurochirurgica·Hugo ArdaillonEkkehard M Kasper
Jun 28, 2014·Cell Transplantation·Zelin ChenChunmeng Shi
May 13, 2014·Cell Transplantation·Tzu-Min ChanShinn-Zong Lin
Feb 11, 2010·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Ralf DresselWolfgang Engel
Aug 29, 2020·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Chenyu Huang, Rei Ogawa
Feb 23, 2020·Stem Cell Research & Therapy·Subramanian ArjunanChui Yee Fong
Jun 13, 2020·Frontiers in Cell and Developmental Biology·Grace C LimandjajaSusan Gibbs
Jul 1, 2013·Plastic and Reconstructive Surgery. Global Open·Chenyu HuangRei Ogawa
Nov 20, 2020·Experimental Dermatology·Edward J MacarakJouni Uitto
Feb 14, 2020·JPRAS Open·Ferdinand W Nangole, George W Agak

❮ Previous
Next ❯

Related Concepts

Related Feeds

Allogenic & Autologous Therapies

Allogenic therapies are generated in large batches from unrelated donor tissues such as bone marrow. In contrast, autologous therapies are manufactures as a single lot from the patient being treated. Here is the latest research on allogenic and autologous therapies.

Adult Stem Cells

Adult stem cells reside in unique niches that provide vital cues for their survival, self-renewal, and differentiation. They hold great promise for use in tissue repair and regeneration as a novel therapeutic strategies. Here is the latest research.

Astrocytes

Astrocytes are glial cells that support the blood-brain barrier, facilitate neurotransmission, provide nutrients to neurons, and help repair damaged nervous tissues. Here is the latest research.