PMID: 138140Jan 1, 1977

Isolation and characterization of muscle membranes using surface-specific labels

Proceedings of the National Academy of Sciences of the United States of America
R L BarchiM Wong

Abstract

Membranes were purified from rat muscle by a differential centrifugation method that avoids the use of salt extraction or incubations at elevated temperature. Three populations of membrane-limited vesicles were defined having average densities of 1.112 (fraction I), 1.141 (fraction II), and 1.158 (fraction III) g/ml in a continuous sucrose gradient. Lactoperoxidase-catalyzed iodination of intact muscle prior to isolation of membranes resulted in highest specific activity in fraction I, although all fractions could be equally labeled after isolation. 125I-Labeled wheat germ agglutinin incubated at low concentration with intact muscle preferentially labeled fraction I. Parallel studies on previously isolated fractions indicated that fraction I also contained the highest concentration of potential receptors for wheat germ agglutinin. In experiments on whole muscle, concanavalin A bound predominantly to sarcolemma with slight variable binding to T-tubular and nuclear membrane but no binding to sarcoplasmic reticulum or mitochondria. Parallel binding studies with isolated membrane fragments indicated heavy binding of concanavalin A by membranes in fraction I with scattered binding in fractions II and III. Na+K+Mg2+ ATPase was specif...Continue Reading

References

Apr 1, 1971·The Journal of Cell Biology·C Franzini-Armstrong
Jul 1, 1969·The Journal of Cell Biology·D W Deamer, R J Baskin
Mar 29, 1973·Biochimica Et Biophysica Acta·A M KidwaiE E Daniel
Jan 1, 1971·Experimental Cell Research·W Bernhard, S Avrameas

Citations

May 1, 1994·Journal of Magnetic Resonance Imaging : JMRI·A G Filler
Apr 1, 1989·Journal of Bioenergetics and Biomembranes·R A Sabbadini, A S Dahms
Aug 1, 1982·Developmental Biology·D Kosk-Kosicka, M G Sarzała
Jan 1, 1979·The International Journal of Biochemistry·A M del Molino, J A Cabezas
Jan 1, 1980·Progress in Biophysics and Molecular Biology·P V Sulakhe, P J St Louis
Jan 21, 2010·BMC Neuroscience·A G FillerAndrew M L Lever
Apr 12, 1979·Biochimica Et Biophysica Acta·K D Philipson, F Baumgartner
Aug 30, 1984·Biochemical and Biophysical Research Communications·E RockJ Chevallier
Feb 1, 1978·Journal of Ultrastructure Research·L M Crowe, R J Baskin
Sep 1, 1979·Experimental Cell Research·N N Malouf, G Meissner
Jun 1, 1990·Archives of Biochemistry and Biophysics·P Wetzel, G Gros
Feb 15, 1985·Archives of Biochemistry and Biophysics·V R OkamotoR A Sabbadini
Jul 1, 1980·Biochimica Et Biophysica Acta·I WallisS Rose
May 17, 1979·Biochimica Et Biophysica Acta·H J MisselwitzA Wollenberger
Sep 19, 1977·Proceedings of the Royal Society of London. Series B, Containing Papers of a Biological Character·D C GadsbyD C Ogden
Sep 25, 2003·Physiological Reviews·Torben Clausen

Related Concepts

DNA-dependent ATPase
Affinity Labels
Cell Fractionation
Centrifugation, Density Gradient
Concanavalin A
Lactoperoxidase
Electron Microscopy
Sarcolemma
Animal Lectins

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