Isolation and characterization of new fission yeast cytokinesis mutants.

Genetics
M BalasubramanianK L Gould

Abstract

Schizosaccharomyces pombe is an excellent organism in which to study cytokinesis as it divides by medial fission using an F-actin contractile ring. To enhance our understanding of the cell division process, a large genetic screen was carried out in which 17 genetic loci essential for cytokinesis were identified, 5 of which are novel. Mutants identifying three genes, rng3(+), rng4(+), and rng5(+), were defective in organizing an actin contractile ring. Four mutants defective in septum deposition, septum initiation defective (sid)1, sid2, sid3, and sid4, were also identified and characterized. Genetic analyses revealed that the sid mutants display strong negative interactions with the previously described septation mutants cdc7-24, cdc11-123, and cdc14-118. The rng5(+), sid2(+), and sid3(+) genes were cloned and shown to encode Myo2p (a myosin heavy chain), a protein kinase related to budding yeast Dbf2p, and Spg1p, a GTP binding protein that is a member of the ras superfamily of GTPases, respectively. The ability of Spg1p to promote septum formation from any point in the cell cycle depends on the activity of Sid4p. In addition, we have characterized a phenotype that has not been described previously in cytokinesis mutants, namel...Continue Reading

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Citations

Jun 20, 2008·Molecular Biology of the Cell·Hongyan YanMohan K Balasubramanian
Mar 10, 2005·Eukaryotic Cell·Mithilesh MishraMohan K Balasubramanian
Jun 16, 2004·The Journal of Biological Chemistry·Samuel J BichselBrian A Hemmings
Jun 26, 2008·The Journal of Biological Chemistry·Erin M NeidtDavid R Kovar
Jan 25, 2002·Journal of Cellular Physiology·Katya RavidMatthew R Jones
Nov 1, 2006·Trends in Biochemical Sciences·Pontus AspenströmNinna Richnau
Sep 30, 2003·Journal of Cell Science·Viesturs Simanis
Feb 21, 2002·Molecular Biology of the Cell·Hongyan WangMohan K Balasubramanian
Apr 12, 2005·Eukaryotic Cell·Andrea BimbóJianhua Liu
Feb 14, 2006·The Journal of Biological Chemistry·Sapna Mehta, Kathleen L Gould
Sep 3, 2004·Current Biology : CB·Andrea KrappViesturs Simanis
Sep 24, 2004·Current Biology : CB·Mohan K BalasubramanianMichael Glotzer
Nov 1, 2000·Nature Cell Biology·N I NaqviM K Balasubramanian
Oct 2, 2002·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·Yabin LuTakayoshi Kuno
Oct 3, 2002·Journal of Cell Science·Alex H HutagalungHenry F Epstein
Oct 3, 2002·Journal of Cell Science·Maureen G PriceHenry F Epstein
May 13, 2006·Current Genetics·Szu Shien NgChristopher J McInerny
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Jan 11, 2008·Molecular Biology of the Cell·Aleksandar VjesticaSnezhana Oliferenko
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Apr 11, 2002·Cell Structure and Function·T NoguchiI Mabuchi

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