Isolation and characterization of novel murine epiphysis derived mesenchymal stem cells.

PloS One
Chun-Chun ChengShinn-Chih Wu

Abstract

While bone marrow (BM) is a rich source of mesenchymal stem cells (MSCs), previous studies have shown that MSCs derived from mouse BM (BMMSCs) were difficult to manipulate as compared to MSCs derived from other species. The objective of this study was to find an alternative murine MSCs source that could provide sufficient MSCs. In this study, we described a novel type of MSCs that migrates directly from the mouse epiphysis in culture. Epiphysis-derived MSCs (EMSCs) could be extensively expanded in plastic adherent culture, and they had a greater ability for clonogenic formation and cell proliferation than BMMSCs. Under specific induction conditions, EMSCs demonstrated multipotency through their ability to differentiate into adipocytes, osteocytes and chondrocytes. Immunophenotypic analysis demonstrated that EMSCs were positive for CD29, CD44, CD73, CD105, CD166, Sca-1 and SSEA-4, while negative for CD11b, CD31, CD34 and CD45. Notably, EMSCs did not express major histocompatibility complex class I (MHC I) or MHC II under our culture system. EMSCs also successfully suppressed the proliferation of splenocytes triggered by concanavalin A (Con A) or allogeneic splenocytes, and decreased the expression of IL-1, IL-6 and TNF-α in Con ...Continue Reading

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Citations

Feb 19, 2016·Development, Growth & Differentiation·Mijin KimByoung-Hyun Min
Jan 29, 2013·Cytotherapy·Veena KrishnappaDonald G Phinney
May 27, 2015·The Journal of Clinical Investigation·Lukas F MagerPhilippe Krebs
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Apr 30, 2015·PloS One·Patricia de Godoy BuenoÂngela Merice de Oliveira Leal
Oct 10, 2018·European Journal of Endocrinology·Tareck Rharass, Stéphanie Lucas
Jan 14, 2018·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Zhen-Yu GuanHsien-Yeh Chen
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Aug 14, 2017·ACS Biomaterials Science & Engineering·Zhen-Yu GuanHsien-Yeh Chen

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Methods Mentioned

BETA
flow cytometry
X-ray
FCS
PCR
Assay

Software Mentioned

ImageJ
FCS Express

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