PMID: 1337Nov 1, 1975

Isolation and characterization of pepsin-treated type III collagen from calf skin

Hoppe-Seyler's Zeitschrift für physiologische Chemie
T Fujii, K Kühn


Calf skin collagen was solubilized by incubating acid-extracted calf skin with pepsin at pH 2.0 and 25 degrees C, conditions that did not cause degradation of the triple helical region of collagen. Type III collagen was separated from type I collagen by differential salt precipitation at pH 7.5. The isolated type III collagen contained mainly gamma and higher molecular weight components cross-linked by reducible and/or non-reducible bonds. The isolated alpha1 (III) chains had an amino acid composition characteristic of type III collagen. Denatured but unreduced type III collagen, chromatographed on carboxymethyl-cellulose, eluted in the alpha 2 region, while after reduction and alkylation the alpha1 (III) chains eluted between the positions of alpha1 (I) and alpha2. The mid-point melting temperature temperature (tm) of type III collagen (35.1 degrees C) in a citrate buffer at pH 3.7 was somewhat lower than that of type I collagen (35.9 degrees C). Renaturation experiments at 25 degrees C showed that denatured type III collagen molecules with intact intramolecular disulfide bridges (gamma components) reform the triple helical structure of collagen much faster than reduced and carboxymethylated alpha1 (III) chains.


Aug 16, 1978·Albrecht Von Graefes Archiv Für Klinische Und Experimentelle Ophthalmologie. Albrecht Von Graefe's Archive for Clinical and Experimental Ophthalmology·O Schmut
Dec 12, 1977·Archives of Dermatological Research·S MatsubayashiS Sano
Jul 21, 1977·Archives of Dermatological Research·W N MeigelL Weber
Jul 15, 1991·Experientia·T D NguyenF-X Maquart
Mar 1, 1994·Biulleten' eksperimental'noĭ biologii i meditsiny·Iu V NesvizhskiĭA A Vorob'ev
Jul 1, 1976·Clinica Chimica Acta; International Journal of Clinical Chemistry·M AdamZ Olsovská
Nov 1, 1983·Collagen and Related Research·M A Gibson, E G Cleary
Jun 1, 1987·Collagen and Related Research·M L SperanzaA Calligaro
Jun 1, 1991·Matrix : Collagen and Related Research·C G Cockburn, M J Barnes
Jan 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·P P FietzekE Wachter
Jan 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·F FauvelJ P Caen
Apr 16, 1984·European Journal of Biochemistry·P Bruckner, E F Eikenberry
Dec 15, 1988·European Journal of Biochemistry·R DölzK Kühn
Jul 1, 1979·Journal of Clinical Chemistry and Clinical Biochemistry. Zeitschrift Für Klinische Chemie Und Klinische Biochemie·M AdamZ Deyl
Jan 1, 1980·Connective Tissue Research·S Tajima, Y Nagai
Nov 15, 1988·Analytical Biochemistry·G BellonF Chastang
Jul 14, 1978·Biochemical and Biophysical Research Communications·J ChandraRajan
Oct 31, 1980·Biochemical and Biophysical Research Communications·Y LegrandJ P Caen
Nov 1, 1979·Experimental Eye Research·R PrausM Adam
Oct 1, 1980·Experimental Eye Research·T T AndreassenH Oxlund
Mar 1, 1983·Biochemistry·S GerardW M Mitchell
Apr 24, 2008·Journal of Biomedical Materials Research. Part a·N ShanmugasundaramMary Babu
Nov 1, 1986·Experimental Cell Research·P GilleryJ P Borel
Apr 9, 2004·Journal of Biomaterials Applications·Natesan ShanmugasundaramMary Babu
Feb 10, 1995·The Journal of Biological Chemistry·E SchönherrH Kresse
Apr 4, 2002·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·E GarbuzenkoF Levi-Schaffer
Aug 31, 2004·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Pascal MauriceFrançoise Fauvel-Lafeve
Feb 1, 1987·Journal of Biomedical Materials Research·M Nagelschmidt, B Viell
Nov 10, 2005·Journal of Biomedical Materials Research. Part B, Applied Biomaterials·N ShanmugasundaramMary Babu
Jun 1, 1977·Journal of the Science of Food and Agriculture·A J Bailey, T J Sims
Oct 1, 1982·European Journal of Clinical Investigation·D P ParrottG C Fuller
Dec 31, 1977·Albrecht Von Graefes Archiv Für Klinische Und Experimentelle Ophthalmologie. Albrecht Von Graefe's Archive for Clinical and Experimental Ophthalmology·H Hofmann, O Schmut
Mar 1, 1983·International Journal of Radiation Biology and Related Studies in Physics, Chemistry, and Medicine·G Boguta, A M Dancewicz

Related Concepts

Bos indicus
Hydrogen-Ion Concentration
Electron Microscopy
Pepsin 3
Plasma Protein Binding Capacity
Protein Conformation
Protein Denaturation

Trending Feeds


Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Alzheimer's Disease: MS4A

Variants within the membrane-spanning 4-domains subfamily A (MS4A) gene cluster have recently been implicated in Alzheimer's disease in genome-wide association studies. Here is the latest research on Alzheimer's disease and MS4A.

Pediculosis pubis

Pediculosis pubis is a disease caused by a parasitic insect known as Pthirus pubis, which infests human pubic hair, as well as other areas with hair including eye lashes. Here is the latest research.

Rh Isoimmunization

Rh isoimmunization is a potentially preventable condition that occasionally is associated with significant perinatal morbidity or mortality. Discover the latest research on Rh Isoimmunization here.

Genetic Screens in iPSC-derived Brain Cells

Genetic screening is a critical tool that can be employed to define and understand gene function and interaction. This feed focuses on genetic screens conducted using induced pluripotent stem cell (iPSC)-derived brain cells. It also follows CRISPR-Cas9 approaches to generating genetic mutants as a means of understanding the effect of genetics on phenotype.

Enzyme Evolution

This feed focuses on molecular models of enzyme evolution and new approaches (such as adaptive laboratory evolution) to metabolic engineering of microorganisms. Here is the latest research.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Pharmacology of Proteinopathies

This feed focuses on the pharmacology of proteinopathies - diseases in which proteins abnormally aggregate (i.e. Alzheimer’s, Parkinson’s, etc.). Discover the latest research in this field with this feed.

Alignment-free Sequence Analysis Tools

Alignment-free sequence analyses have been applied to problems ranging from whole-genome phylogeny to the classification of protein families, identification of horizontally transferred genes, and detection of recombined sequences. Here is the latest research.