Isolation and characterization of peroxisome-deficient Chinese hamster ovary cell mutants representing human complementation group III

Experimental Cell Research
K OkumotoY Fujiki

Abstract

We made use of the 9-(1'-pyrene)nonanol/ultraviolet (P9OH/UV) method and isolated peroxisome-deficient mutant cells. TKa cells, the wild-type Chinese hamster ovary (CHO) cells, CHO-K1, that had been stably transfected with cDNA encoding Pex2p (formerly peroxisome assembly factor-1, PAF-1) were used to avoid frequent isolation of the Z65-type, PEX2-defective mutants. P9OH/UV-resistant cell colonies were examined for the intracellular location of catalase, a peroxisomal matrix enzyme, by immunofluorescence microscopy and using anti-catalase antibody. As six mutant cell clones showed cytosolic catalase, there was likely to be a deficiency in peroxisome assembly. These mutants also showed the typical peroxisome assembly-defective phenotype, including significant decrease of dihydroxyacetonephosphate acyltransferase, the first step key enzyme in plasmalogen synthesis, and loss of resistance to 12-(1'-pyrene)dodecanoic acid/UV treatment. By transfection of Pex2p and Pex6p (formerly PAF-2) cDNAs and cell fusion analysis between the CHO cell mutants, two mutants, ZP104 and ZP109, were found to belong to a novel complementation group. Further complementation analysis using fibroblasts from patients with peroxisome biogenesis disorders r...Continue Reading

References

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Citations

Jun 10, 2000·Molecular Biology of the Cell·K GhaediY Fujiki
May 16, 1998·Proceedings of the National Academy of Sciences of the United States of America·S TamuraY Fujiki
Mar 3, 1999·Proceedings of the National Academy of Sciences of the United States of America·Y MatsuzonoY Fujiki
Nov 14, 1997·Nature Genetics·K Okumoto, Y Fujiki
Apr 12, 2013·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·Masafumi NoguchiYukio Fujiki
May 11, 2011·Traffic·Kanji OkumotoYukio Fujiki
Jun 11, 2002·Biochemical and Biophysical Research Communications·Noriko AkiyamaYukio Fujiki
May 26, 2006·Biochemical and Biophysical Research Communications·Wei Fan, Yukio Fujiki
Jun 11, 2002·Biochemical and Biophysical Research Communications·Eiko YanagoYukio Fujiki
May 20, 1998·Biochemical and Biophysical Research Communications·S TamuraY Fujiki
Dec 31, 1997·Biochimica Et Biophysica Acta·Y Fujiki
Nov 25, 1998·Biochemical and Biophysical Research Communications·I Abe, Y Fujiki
Oct 19, 2006·Biochimica Et Biophysica Acta·Yukio FujikiKamran Ghaedi
Sep 7, 2016·The Journal of Cell Biology·Graeme SargentPeter Kijun Kim
Sep 12, 2002·The Journal of Biological Chemistry·Masanori HonshoYukio Fujiki
Oct 11, 2013·The Journal of Biological Chemistry·Masanori HonshoYukio Fujiki
Oct 16, 2015·The Journal of Biological Chemistry·Masanori HonshoYukio Fujiki
Jul 24, 2003·Journal of Cell Science·Jörg H Eckert, Nils Johnsson

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