Isolation and characterization of polycyclic aromatic hydrocarbons-degrading Sphingomonas sp. strain ZL5

Biodegradation
Yongsheng LiuZhongze Zhang

Abstract

A bacterial strain ZL5, capable of growing on phenanthrene as a sole carbon and energy source but not naphthalene, was isolated by selective enrichment from crude-oil-contaminated soil of Liaohe Oil Field in China. The isolate was identified as a Sphingomonas sp. strain on the basis of 16S ribosomal DNA analysis. Strain ZL5 grown on phenanthrene exhibited catechol 2,3-dioxygenase (C23O) activity but no catechol 1,2-dioxygenase, gentisate 1,2-dioxygenase, protocatechuate 3,4-dioxygenase and protocatechuate 4,5-dioxygenase activities. This suggests that the mode of cleavage of phenanthrene by strain ZL5 could be meta via the intermediate catechol, which is different from the protocatechuate way of other two bacteria, Alcaligenes faecelis AFK2 and Nocardioides sp. strain KP7, also capable of growing on phenanthrene but not naphthalene. A resident plasmid (approximately 60 kb in size), designated as pZL, was detected from strain ZL5. Curing the plasmid with mitomycin C and transferring the plasmid to E. coli revealed that pZL was responsible for polycyclic aromatic hydrocarbons degradation. The C23O gene located on plasmid pZL was cloned and overexpressed in E. coli JM109(DE3). The ring-fission activity of the purified C23O from th...Continue Reading

Citations

Aug 30, 2008·Applied Microbiology and Biotechnology·Ping ZengJoo-Hwa Tay
Nov 13, 2008·Applied Microbiology and Biotechnology·Andreas Stolz
Nov 17, 2009·Journal of Bioscience and Bioengineering·Piriya KlankeoOnruthai Pinyakong
Aug 10, 2013·Marine Pollution Bulletin·Wanwasan WongwongseeOnruthai Pinyakong
Nov 30, 2018·Microbial Ecology·Aleksandra S ZakharenkoTamara I Zemskaya
Nov 18, 2018·The Science of the Total Environment·Klaudia KosekŻaneta Polkowska

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