Isolation and characterization of polymorphic nuclear microsatellite loci in Pinus cembra L

Molecular Ecology Resources
K SalzerG G Vendramin

Abstract

We developed eight polymorphic nuclear microsatellite markers for the Swiss stone pine (Pinus cembra L.), of which seven may be amplified in a multiplex polymerase chain reaction. Allelic polymorphism across all loci and 40 individuals representing two populations in the Swiss Alps was high (mean = 7.6 alleles). No significant linkage disequlibrium was displayed between pairs of loci. Significant deviation from Hardy-Weinberg equilibrium was revealed at three loci in one population. Cross-amplification was achieved in two related species within the genus (P. sibirica and P. pumila). Thus, the markers may be useful for population genetic studies in these three pine species. They will be applied in ongoing projects on genetic diversity and patterns of gene flow in P. cembra.

References

Mar 20, 1999·Molecular Phylogenetics and Evolution·A ListonE R Alvarez-Buylla
Feb 27, 2004·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·S C González-MartínezM T Cervera
Oct 19, 2004·Molecular Ecology·Paula E Marquardt, Bryan K Epperson
Jan 1, 2008·Molecular Ecology Resources·François Rousset

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