PMID: 8602463Mar 1, 1996Paper

Isolation and characterization of porcine mannan-binding proteins of different size and ultrastructure

Scandinavian Journal of Immunology
P StorgaardS E Svehag

Abstract

The authors report on the purification and characterization of mannan- binding proteins (MBP) isolated from porcine serum. The MBPs were purified by use of PEG precipitation, affinity chromatography on mannan-Sepharose, protein A- and anti-porcine IgM-Sepharose followed by gel filtration. The MBP proteins were collagenase sensitive and showed gamma 1-gamma 2-electrophoretic mobility. The MBP designated pMBP-28 had a molecular mass of 28 kDa when analysed on SDS-PAGE under reducing conditions and eluted corresponding to a molecular mass of approximately 700 kDa on gel filtration chromatography. Electron micrographs of pMBP-28 revealed an oligomeric protein similar to rodent MBP-A and human MBP but with a predominance of penta- and hexameric molecules. Another protein designated pMBP-27 was composed of peptides of 27 kDa and had an Mr of 300-350 kDa on gel filtration chromatography. Electron microscopy of pMBP-27 showed dimer and trimer molecules; the trimers without distinct stalk regions. The N-terminal 26(pMBP-27) and 24(MBP-28) amino acid residues showed 54% and 58% identity with human MBP.pMBP-28 showed a higher degree of sequence similarity to rat and mouse MBP-A (60% identity) than to mouse and rat MBP-C (41-45% identity)....Continue Reading

Citations

Nov 8, 2006·Immunogenetics·Brandon N LillieM Anthony Hayes
Jun 15, 2012·Marine Biotechnology·Aleksandr A BulgakovValery A Rasskazov
Mar 16, 2000·Developmental and Comparative Immunology·S B Laursen, O L Nielsen
Oct 6, 2000·APMIS. Supplementum·U L Holmskov
May 12, 2007·Veterinary Immunology and Immunopathology·Lise R PhaneufPatricia V Turner
Dec 30, 2006·Developmental and Comparative Immunology·Brandon N LillieM Anthony Hayes
Oct 16, 2004·Genes and Immunity·M V Verga FalzacappaS Crovella
Dec 9, 2003·The Journal of Biological Chemistry·Tomoo Ohashi, Harold P Erickson

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