Isolation and characterization of porcine deltacoronavirus from pigs with diarrhea in the United States

Journal of Clinical Microbiology
Hui HuL J Saif

Abstract

Porcine deltacoronavirus (PDCoV) is a novel coronavirus that causes diarrhea in nursing piglets. Following its first detection in the United States in February 2014, additional PDCoV strains have been identified in the United States and Canada. Currently, no treatments or vaccines for PDCoV are available. In this study, U.S. PDCoV strain OH-FD22 from intestinal contents of a diarrheic pig from Ohio was isolated in swine testicular (ST) and LLC porcine kidney (LLC-PK) cell cultures by using various medium additives. We also isolated PDCoV [OH-FD22(DC44) strain] in LLC-PK cells from intestinal contents of PDCoV OH-FD22 strain-inoculated gnotobiotic (Gn) pigs. Cell culture isolation and propagation were optimized, and the isolates were serially propagated in cell culture for >20 passages. The full-length S and N genes were sequenced to study PDCoV genetic changes after passage in Gn pigs and cell culture (passage 11 [P11] and P20). Genetically, the S and N genes of the PDCoV isolates were relatively stable during the first 20 passages in cell culture, with only 5 nucleotide changes, each corresponding to an amino acid change. The S and N genes of our sequenced strains were genetically closely related to each other and to other U.S...Continue Reading

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Citations

Mar 18, 2016·Expert Review of Vaccines·Lanying DuShibo Jiang
Mar 13, 2016·Transboundary and Emerging Diseases·J H LeeB K Park
Dec 21, 2016·The Journal of General Virology·Puxian FangShaobo Xiao
Sep 1, 2016·Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·Sarah Vitosh-SillmanRichard Hesse
May 16, 2018·Proceedings of the National Academy of Sciences of the United States of America·Wentao LiBerend-Jan Bosch
May 27, 2016·Journal of Clinical Microbiology·Yunfeng SongSheela Ramamoorthy
Apr 4, 2019·Transboundary and Emerging Diseases·Claudia Pérez-RiveraJosé Ivan Sánchez-Betancourt
Dec 6, 2019·Journal of Virology·Vineet D MenacheryRalph S Baric
Feb 25, 2020·Emerging Microbes & Infections·Yue-Lin YangYan-Dong Tang
May 19, 2020·Veterinary Medicine and Science·Haixin HuangNingyi Jin
May 15, 2020·Molecular Biology and Evolution·Wan-Ting HeShuo Su
Jun 27, 2019·Viruses·Qingqing LiangHui Hu
Apr 27, 2018·Journal of Veterinary Science·Guehwan JangChanghee Lee
Mar 12, 2019·Frontiers in Veterinary Science·Surapong KoonpaewAnan Jongkaewwattana
Jan 22, 2020·Emerging Infectious Diseases·Patricia A BoleyScott P Kenney
Feb 13, 2020·Revista Da Sociedade Brasileira De Medicina Tropical·Mara Lucia GravinattiFábio Gregori
Jan 16, 2020·Journal of Veterinary Science·Yunfei ZhangHui Hu

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