Isolation and characterization of progenitor cells from surgically created early healing alveolar defects in humans: A preliminary study

Journal of Periodontology
Adriana Campos Passanezi Sant'AnaEuloir Passanezi

Abstract

The granulation tissue present in surgically-created early healing sockets has been considered as a possible source of osteoprogenitor cells for periodontal regeneration, as demonstrated in animal studies. However, the in vitro osteogenic properties of tissue removed from human surgically-created early healing alveolar defects (SC-EHAD) remains to be established, being that the aim of this study. Surgical defects were created in the edentulous ridge of two systemically healthy adults. The healing tissue present in these defects was removed 21 days later for the establishment of primary culture. The in vitro characteristics of the cultured cells were determined by Armelin method, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, immunohistochemistry, alkaline phosphatase (ALP) activity, mineralization assay, and flow cytometry for detection of stem cells/osteoprogenitor cell markers. Cells were able to adhere to the plastic and assumed spindle-shaped morphology at earlier passages, changing to a cuboidal one with increasing passages. Differences in the proliferation rate were observed with increasing passages, suggesting osteogenic differentiation. ALP and mineralization activities were detected in conven...Continue Reading

References

Mar 1, 1979·Journal of Periodontology·S E Soehren, R L Van Swol
Jul 1, 1986·Journal of Periodontal Research·U M WikesjöV P Terranova
Mar 1, 1986·Calcified Tissue International·C G BellowsM E Antosz
May 1, 1983·The American Journal of Anatomy·C A McCulloch, A H Melcher
Feb 1, 1982·Journal of Periodontology·C I EvianH Corn
Oct 16, 1999·Journal of Clinical Periodontology·M G AraújoJ Lindhe
Jan 11, 2003·International Journal of Oral and Maxillofacial Surgery·H Devlin, P Sloan
Sep 6, 2003·Journal of Clinical Periodontology·G CardaropoliJ Lindhe
Sep 11, 2003·Journal of Oral and Maxillofacial Surgery : Official Journal of the American Association of Oral and Maxillofacial Surgeons·Zahid LalaniP J Duke
Jul 1, 1961·Oral Surgery, Oral Medicine, and Oral Pathology·M H AMLER, P L JOHNSON
Jul 1, 1960·The Journal of the American Dental Association·M H AMLERI SALMAN
Mar 5, 2005·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Takehiro MatsubaraYukio Kato
Aug 4, 2012·Journal of Applied Oral Science : Revista FOB·Adriana Campos Passanezi Sant'anaEuloir Passanezi
Aug 30, 2014·Journal of Dental Research·R NakajimaT Kuboki
Sep 13, 2014·Journal of Periodontology·Richard T KaoMark A Reynolds
Jun 11, 2015·Journal of Cellular Physiology·Seyed Hossein BassirGiuseppe Intini

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Citations

Mar 14, 2019·Journal of Periodontal Research·Bruna F R FerrazAdriana C P Sant'Ana
Jun 26, 2020·Journal of Dental Research·D ZhangQ Yuan
Aug 26, 2020·International Journal of Implant Dentistry·Maria Alejandra Frias MartinezAdriana Campos Passanezi Sant'Ana

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