Abstract
Although the highly proliferative, migratory and multidrug resistant phenotype of human type II endometrial carcinoma (EC) is well characterized, improved clinical treatments have not yet been developed. In this study, CD44 and CD133 were used as markers to screen, isolate and enrich carcinoma-initiating cells (CICs) from the human type II EC cell lines KLE and ANCA3. Using flow cytometry, we identified a subpopulation of KLE and ANCA3 cells which express high levels of both CD44 and CD133 on the cell membrane. CD44+/CD133+ EC-CICs exhibited high levels of stemness marker genes, and possessed a higher migratory and invasive ability than CD44-/CD133- endometrial carcinoma cells (EC-CCs). CD44+/CD133+ EC-CICs were also more resistant to growth inhibition induced by the chemotherapeutic drugs cisplatin and paclitaxel. Additionally, CD44+/CD133+ EC-CICs readily established tumors in vivo in a relatively short time. In conclusion, CD44+/CD133+ cells possessing the characteristics of CICs can be reliably sorted from KLE and ANCA3 human type II EC cell lines, and represent a valuable model for studying cancer cell physiology and multidrug resistance. Further characterization of CD44+/CD133+ KLE and ANCA3 cells may have a profound impa...Continue Reading
References
Feb 1, 1983·Gynecologic Oncology·J V Bokhman
Feb 17, 1994·Nature·T LapidotJ E Dick
Jul 1, 1997·Nature Medicine·D Bonnet, J E Dick
Nov 2, 2001·Nature·T ReyaI L Weissman
Mar 12, 2003·Proceedings of the National Academy of Sciences of the United States of America·Muhammad Al-HajjMichael F Clarke
Sep 6, 2003·Science·Jean Marx
Jan 23, 2004·Nature Reviews. Cancer·Ricardo PardalSean J Morrison
Nov 19, 2004·Nature·Sheila K SinghPeter B Dirks
Jul 5, 2005·Cancer Research·Dario PontiMaria Grazia Daidone
Nov 24, 2006·Nature·Lucia Ricci-VitianiRuggero De Maria
Nov 24, 2006·Nature·Catherine A O'BrienJohn E Dick
Feb 27, 2007·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·Elena MonzaniCaterina A M La Porta
Jun 6, 2007·Proceedings of the National Academy of Sciences of the United States of America·Piero DalerbaMichael F Clarke
Dec 1, 2007·Cell Death and Differentiation·A EramoR De Maria
Jun 4, 2008·Cancer Research·Shu ZhangKenneth P Nephew
Aug 1, 2009·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Jean-François MayolDidier Wion
Nov 12, 2009·Gynecologic Oncology·Paola A Gehrig, Victoria L Bae-Jump
Mar 13, 2010·Molecular and Cellular Biochemistry·Te LiuZhixue Liu
Apr 16, 2010·Nature·Rajnish A GuptaHoward Y Chang
Aug 14, 2010·Acta Biochimica Et Biophysica Sinica·Li MaLihe Guo
Mar 11, 2011·Nature Reviews. Cancer·Margot Zöller
Oct 6, 2011·BMC Cancer·Feizhou JiangXiaoping Wan
Citations
Dec 22, 2014·FEBS Letters·Yongtao GaoYongyi Huang
Jan 24, 2013·Stem Cells·Deanna M JanzenSanaz Memarzadeh
Jul 18, 2017·Oncology Reports·Yan-Cui GongHai-Hua Yu
Oct 31, 2019·Scientific Reports·Yan ZhuPeter C K Leung
Apr 27, 2021·Frontiers in Cell and Developmental Biology·Fiona L CousinsCaroline E Gargett
Jun 3, 2021·Journal of Clinical Medicine·Milosz PietrusKlaudia Skrzypek