Isolation and characterization of promoters from the Lactobacillus casei temperate bacteriophage A2

Canadian Journal of Microbiology
P GarcíaJ E Suárez

Abstract

Random Sau3A1 DNA fragments from the temperate Lactobacillus bacteriophage A2 were cloned into the promoter-probe plasmid pGKV210. Seven DNA fragments with promoter activity were selected, after transformation of Escherichia coli and Lactococcus lactis, subsp, lactis, through the chloramphenicol resistance they conferred to the corresponding clones. The seven promoters were functional in Lactobacillus casei. Their strength was analysed by measuring the levels of chloramphenicol resistance and chloramphenicol acetyltransferase activity induced in each host. The nucleotide sequences of these fragments were determined and primer extension analysis was used to locate the initiation site of transcription from each promoter in E coli. The promoters contained -10 and -35 regions similar to the consensus sequences of E. coli and Lactobacillus promoters.

References

Dec 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·F SangerA R Coulson
Nov 24, 1979·Nucleic Acids Research·H C Birnboim, J Doly
Feb 1, 1991·Applied and Environmental Microbiology·T KoivulaI Palva
May 1, 1991·Applied and Environmental Microbiology·P SlosA Mercenier
Oct 1, 1987·Applied and Environmental Microbiology·J M van der VossenG Venema
Jul 11, 1988·Nucleic Acids Research·W J DowerC W Ragsdale
Feb 1, 1986·Analytical Biochemistry·M Hattori, Y Sakaki
Apr 25, 1983·Nucleic Acids Research·D K Hawley, W R McClure
Jan 11, 1984·Nucleic Acids Research·J DevereuxO Smithies
Sep 15, 1994·FEMS Microbiology Letters·A Constable, B Mollet
Jan 1, 1993·Antonie van Leeuwenhoek·P H Pouwels, R J Leer
Oct 1, 1996·Antonie van Leeuwenhoek·B E DavidsonA J Hillier
Sep 1, 1990·Applied and Environmental Microbiology·K J LeenhoutsG Venema
Aug 1, 1993·Applied and Environmental Microbiology·D J O'sullivan, T R Klaenhammer

❮ Previous
Next ❯

Citations

Jun 10, 2008·Applied and Environmental Microbiology·Evelyn DurmazTodd R Klaenhammer
May 7, 2003·Applied and Environmental Microbiology·Trine NilsenHelge Holo
Nov 9, 2000·Canadian Journal of Microbiology·S Arsenijevic, L Topisirovic

❮ Previous
Next ❯

Related Concepts

Related Feeds

Bacteriophage: Phage Therapy

Phage therapy uses bacterial viruses (bacteriophages) to treat bacterial infections and is widely being recognized as an alternative to antibiotics. Here is the latest research.