PMID: 7545151Sep 1, 1995Paper

Isolation and characterization of ropA homologous genes from Rhizobium leguminosarum biovars viciae and trifolii

Journal of Bacteriology
H P RoestB J Lugtenberg

Abstract

ropA encodes a 36-kDa outer membrane protein of Rhizobium leguminosarum bv. viciae strain 248 which constitutes the low-M(r) part of antigen group III (R.A. de Maagd, I.H.M. Mulders, H.C.J. Canter Cremers, B.J.J. Lugtenberg, J. Bacteriol. 174:214-221, 1992). We observed that genes homologous to ropA are present in strain 248 as well as in other R. leguminosarum strains, and we describe the cloning and characterization of two of these genes. Sequencing of a 2.2-kb Bg/II fragment from R. leguminosarum bv. viciae strain 248 that hybridizes with ropA revealed one large open reading frame of 1,074 bp encoding a mature protein of 38.096 kDa. Homology between this gene and ropA is 91.8% on the DNA level. Homology on the amino acid level is only 69.9% as a result of a frameshift. On the basis of homology and immunochemical characteristics, we conclude that this gene encodes the high-M(r) part of the outer membrane protein antigen group III that is repressed during symbiosis. We named this gene ropA2. The second gene that we cloned was the ropA homologous gene of R. leguminosarum bv. trifolii strain LPR5020. Except for amino acid 43, the N-terminal part of the corresponding protein appeared to be identical to the first 51 amino acids of...Continue Reading

References

Dec 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·F SangerA R Coulson
Jul 1, 1991·Journal of Bacteriology·H R SchlamanB J Lugtenberg
Jan 1, 1988·Annual Review of Biochemistry·J D Helmann, M J Chamberlin
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Citations

Nov 15, 1996·FEMS Microbiology Letters·A CloeckaertN Vizcaíno
Jun 12, 2013·Journal of Bacteriology·Matthew B CrookJoel S Griffitts
Nov 19, 2015·Frontiers in Microbiology·Shivani GoolabMichael C Crampton
Sep 10, 2020·Proceedings of the National Academy of Sciences of the United States of America·Rachel M WheatleyPhilip S Poole
Oct 4, 2020·International Journal of Molecular Sciences·Anastasiia O KosolapovaAnton A Nizhnikov
Nov 5, 2002·Veterinary Microbiology·Axel CloeckaertPhilip H Elzer

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