Jun 9, 2020Paper

Isolation and characterization of SARS-CoV-2 from the first US COVID-19 patient

BioRxiv : the Preprint Server for Biology
Jennifer HarcourtNatalie J Thornburg

Abstract

The etiologic agent of the outbreak of pneumonia in Wuhan China was identified as severe acute respiratory syndrome associated coronavirus 2 (SARS-CoV-2) in January, 2020. The first US patient was diagnosed by the State of Washington and the US Centers for Disease Control and Prevention on January 20, 2020. We isolated virus from nasopharyngeal and oropharyngeal specimens, and characterized the viral sequence, replication properties, and cell culture tropism. We found that the virus replicates to high titer in Vero-CCL81 cells and Vero E6 cells in the absence of trypsin. We also deposited the virus into two virus repositories, making it broadly available to the public health and research communities. We hope that open access to this important reagent will expedite development of medical countermeasures.

Citations

Jul 1, 2020·Turkish Journal of Biology = Türk Biyoloji Dergisi·Cihan TaŞtanErcüment Ovali
Apr 2, 2020·ELife·Yinon M Bar-OnRon Milo
May 22, 2020·Indian Journal of Medical Microbiology·Parakriti GuptaMini P Singh
Jan 4, 2021·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·Nathalie WurtzBernard La Scola
Dec 9, 2020·Medical Hypotheses·Isabella Neme Ribeiro ReisClaudio Mendes Pannuti
Oct 1, 2019·Indian Journal of Medical Microbiology·Parakriti GuptaMini P Singh
Nov 6, 2020·Biomédica : revista del Instituto Nacional de Salud·Francisco J DíazMaría Teresa Rugeles
Jan 15, 2021·Clinical Microbiology Reviews·Aleksandra SynowiecKrzysztof Pyrc
Apr 11, 2021·Environmental Science and Pollution Research International·Xiu Wu, Jinting Zhang
May 11, 2021·Frontiers in Pharmacology·G KanimozhiN Rajendra Prasad
Jul 3, 2021·Microorganisms·Rafael K CamposShannan L Rossi
Jun 22, 2021·Frontiers in Medicine·Arjun SharmaMarkus Bosmann
Jul 3, 2021·Scientific Reports·Natalia G SampaioJan Rehwinkel
Jan 21, 2022·Nucleic Acids Research·Ankit K PathakMitali Mukerji

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