Isolation and characterization of six pathogenesis-related (PR) proteins of Samsun NN tobacco

Plant Molecular Biology
S KauffmannB Fritig

Abstract

The purification to homogeneity of pathogenesis-related (PR) proteins R and S from Nicotiana tabacum cv. Samsun NN leaves has been achieved by using a combination of conventional and high-performance chromatographic supports. The same procedure allowed the purification and the characterization of four other proteins which displayed some properties characteristic of tobacco PR proteins and were shown to accumulate in tobacco leaves in response to virus infection. They can be, therefore, considered as new tobacco PR proteins which we designate as PR-s1, -s2, -r1 and -r2. The relative electrophoretic mobilities (Rf) under non-denaturing conditions were estimated to 0.30 for PR-r1 and -r2, 0.25 for Pr-R, 0.20 for PR-s1 and -s2 and 0.15 for PR-S. On SDS gels PR proteins R and S possessed the same apparent molecular weight (Mr 24,000) as did PR-proteins s1 and r1 (Mr 14,500) and PR-s2 and -r2 (Mr 13,000). However, proteins s1, s2, r1 and r2 had identical electrophoretic mobilities on SDS gels when the loading sample buffer contained no reducing agent. Polyclonal antisera were raised against PR proteins R and S and used in immunoblotting experiments. Proteins R and S were shown to be serologically closely related. No cross-reaction wa...Continue Reading

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Citations

Aug 31, 1992·Biochemical and Biophysical Research Communications·J R BorgmeyerQ K Huynh
Nov 26, 1999·The Plant Journal : for Cell and Molecular Biology·M RauscherH B Deising
Jan 24, 2014·Plant Molecular Biology·Shankar R PantVincent P Klink
Dec 29, 2018·PloS One·María José CastellóPablo Tornero

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