PMID: 109328Jan 1, 1979

Isolation and characterization of specific rat epididymal proteins

Molecular and Cellular Endocrinology
J C GarberiJ A Blaquier

Abstract

The partial purification and characterization of specific rat epididymal proteins (SEP) is reported. Starting from the cytosol fraction obtained from epididymal homogenates, protein C was purified 15-fold and proteins D--E were purified 19-fold. The molecular weight, determined by molecular sieving, of protein C was 22,400 while that of D--E was 37,000. These proteins stained as glycoproteins with periodic acid--Schiff reagent. The isoelectric point of protein D was 5.13 while that of protein E was 4.95. Protein C separated into 3 bands during isoelectric focussing. The major component focussed at 5.56 and the two minor components at pH 5.38 And 5.79. Using a specific antiserum we could confirm the organ specificity of SEP and their androgen-dependence.

References

Aug 1, 1978·Biology of Reproduction·G E Olson, David W Hamilton
Aug 1, 1976·The Journal of Experimental Zoology·J M Cummins
Apr 1, 1976·The Journal of Endocrinology·M S Cameo, J A Blaquier
Jul 1, 1969·Analytical Biochemistry·R M ZachariusJ J Woodlock
May 1, 1971·The International Journal of Applied Radiation and Isotopes·J Cadet, R Teoule
Jun 1, 1969·Biology of Reproduction·M C Orgebin-Crist
Feb 1, 1971·Journal of Reproduction and Fertility·U LavonD Danon
Jun 1, 1971·Journal of Reproduction and Fertility·E AlumotH Schindler
Dec 1, 1968·Analytical Biochemistry·N Catsimpoolas

Citations

Jan 1, 1992·Molecular Reproduction and Development·L Rochwerger, Patricia S Cuasnicú
May 1, 1992·Molecular Reproduction and Development·D R Mattmueller, B T Hinton
Jul 1, 1979·Journal of Steroid Biochemistry·A C KohaneJ A Blaquier
Jan 1, 1987·Comparative Biochemistry and Physiology. B, Comparative Biochemistry·A DepeigesJ P Dufaure
Jun 15, 2004·Biochemical and Biophysical Research Communications·Namasivayam ElangovanSin-Tak Chu
Dec 1, 1982·International Journal of Andrology·J C GarberiJ A Blaquier
Sep 30, 2015·The Journal of Cell Biology·Juan I ErnestoPatricia S Cuasnicú
Jun 24, 2008·Developmental Biology·Vanina G Da RosPatricia S Cuasnicú
Aug 3, 2007·Biology of Reproduction·Dolores BussoPatricia S Cuasnicú
Jul 25, 2000·Biology of Reproduction·D J CohenPatricia S Cuasnicú
Jan 5, 2007·Biology of Reproduction·Dolores BussoPatricia S Cuasnicú
Nov 1, 1985·The American Journal of Anatomy·E M EddyB A Fenderson
Sep 1, 1987·The American Journal of Anatomy·M C Orgebin-CristM D Skudlarek
Jun 1, 1981·International Journal of Andrology·P Y WongW M Lee
Nov 1, 1982·Andrologia·J L CourtensS Fournier-Delpech
Jan 1, 1983·American Journal of Primatology·M Z HaiderM Arslan
Aug 1, 1983·Archives of Andrology·A BernalJ J Hicks
May 1, 1986·Journal of Andrology·C J FlickingerK E Ertl

Related Concepts

Epididymis
Immunodiffusion Measurement
Neoglycoproteins
Isoelectric Focusing

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