Isolation and characterization of SUG2. A novel ATPase family component of the yeast 26 S proteasome.

The Journal of Biological Chemistry
S J RussellS A Johnston

Abstract

Using a genetic strategy designed to find proteins involved in the function of the Saccharomyces cerevisiae transcriptional activator GAL4, we isolated mutants in two genes which rescue a class of gal4 activation domain mutants. One of these genes, SUG1, encodes a member of a large family of putative ATPases, the Conserved ATPase containing Domain (CAD) proteins (also known as AAA proteins) that are involved in a wide variety of cellular functions. Subsequently, SUG1 was identified as a subunit of the 26 S proteasome. We have now cloned the gene defined by the second complementation group. SUG2 encodes an essential 49-kDa protein that is also a member of the CAD family and is 43% identical to SUG1. The mutation in sug2-1, like that in sug1-1, is found in the CAD near the highly conserved ATPase motif. We present biochemical and genetic evidence that SUG2 is associated in vivo with SUG1 and is a novel CAD protein subunit of the 26 S proteasome. With its highly conserved mammalian homologs, human p42 and ground squirrel CADp44, SUG2 defines a new class of proteasomal CAD proteins.

References

Jan 1, 1976·Journal of Bacteriology·O M Kew, H C Douglas
May 29, 1992·Science·K K Leuther, S A Johnston
Jan 1, 1991·Methods in Enzymology·I Herskowitz, R E Jensen
May 24, 1991·Science·A LupasJ Stock
Feb 24, 1989·Cell·B J Thomas, R Rothstein
Nov 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·S A Johnston, J E Hopper
Jan 1, 1983·Journal of Bacteriology·H ItoA Kimura
Aug 1, 1995·Current Biology : CB·D M Rubin, D Finley
Jul 1, 1995·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·F Confalonieri, M Duguet
Jan 1, 1993·Enzyme & Protein·A LupasW Baumeister
Mar 1, 1995·Nature Structural Biology·T Wenzel, W Baumeister
Nov 21, 1994·FEBS Letters·M FischerD H Wolf
Jan 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·B OhanaC A Rosen
Feb 9, 1996·The Journal of Biological Chemistry·G N DeMartinoC A Slaughter

❮ Previous
Next ❯

Citations

May 22, 2001·Archives of Biochemistry and Biophysics·R Hartmann-PetersenK B Hendil
Aug 29, 2003·The Journal of Steroid Biochemistry and Molecular Biology·Ming Qiu, Carol A Lange
Aug 8, 1998·Trends in Cell Biology·S Patel, M Latterich
Feb 9, 2000·Trends in Biochemical Sciences·K FerrellC Gordon
Sep 27, 2007·Journal of the American Chemical Society·Bo LiuThomas Kodadek
Mar 4, 2003·Nature Reviews. Molecular Cell Biology·Masafumi Muratani, William P Tansey
May 15, 2010·Molecular & Cellular Proteomics : MCP·Friedrich FörsterWolfgang Baumeister
Sep 12, 2008·Molecular Biology of the Cell·Marianna TcherpakovZe'ev Ronai
May 24, 2001·Molecular Biology and Evolution·K Wollenberg, J C Swaffield
Dec 2, 2005·Genes & Development·Athar Ansari, Michael Hampsey
Jun 19, 2001·Genes & Development·T G GilletteE C Friedberg
Jun 29, 2000·Annual Review of Biochemistry·D VogesW Baumeister
Apr 5, 2005·Archaea : an International Microbiological Journal·B FranzettiG Zaccai
Mar 13, 2012·Annual Review of Biochemistry·Fuqiang GengWilliam P Tansey
Apr 5, 2005·Archaea : an International Microbiological Journal·Greg D Bell, Dennis W Grogan
May 5, 1997·The Journal of Cell Biology·H B McDonald, B Byers
Sep 12, 2014·Biomolecules·Geetha Durairaj, Peter Kaiser
Aug 6, 2009·Biochemical and Biophysical Research Communications·Friedrich FörsterWolfgang Baumeister
Feb 26, 1998·Cell·W BaumeisterE Seemüller
Jan 27, 1998·The Journal of Biological Chemistry·C SearsT Maniatis
Nov 26, 2014·Biomolecules·Tyler S McCann, William P Tansey
Jul 27, 1999·The Journal of Biological Chemistry·S J RussellS A Johnston
Jan 21, 2001·The Journal of Biological Chemistry·S J Russell, S A Johnston
Jun 20, 1998·Molecular and Cellular Biology·M H GlickmanD Finley
Aug 13, 2013·G3 : Genes - Genomes - Genetics·Lance F DaSilvaChristopher J Brandl
Jun 22, 2001·The Journal of Biological Chemistry·C ChangT Kodadek
Feb 25, 1998·Biochemical and Biophysical Research Communications·N TanahashiK Tanaka
Apr 20, 2002·Science·Soren OttosenSteven J Triezenberg

❮ Previous
Next ❯

Related Concepts

Related Feeds

ASBMB Publications

The American Society for Biochemistry and Molecular Biology (ASBMB) includes the Journal of Biological Chemistry, Molecular & Cellular Proteomics, and the Journal of Lipid Research. Discover the latest research from ASBMB here.