PMID: 2115173Jul 1, 1990Paper

Isolation and characterization of sulfhydryl and disulfide peptides of human apolipoprotein B-100

Proceedings of the National Academy of Sciences of the United States of America
C Y YangA M Gotto

Abstract

Twenty-three of the 25 cysteine residues in apolipoprotein B-100 have been isolated directly from tryptic or peptic peptide mixtures. Sixteen cysteine residues exist in disulfide forms: Cys-1-Cys-3, Cys-2-Cys-4, Cys-5-Cys-6, Cys-7-Cys-8, Cys-9-Cys-10, Cys-11-Cys-12, Cys-13-Cys-14, and Cys-20-Cys-21. All of these except Cys-20-Cys-21 are recently discovered disulfide linkages. In addition to Cys-22 and Cys-24, which have been described as sulfhydryls on low density lipoprotein, Cys-15 to Cys-18 and Cys-23 are in the reduced form. Cys-19 and Cys-25 are not yet confirmed. Our results revealed that all identified disulfide linkages are located in the trypsin-releasable regions and that all except Cys-1-Cys-3 and Cys-2-Cys-4 are linked to the neighboring cysteine. We propose a linear model of apolipoprotein B-100 in low density lipoprotein that wraps around the low density lipoprotein molecule.

References

Jan 1, 1989·Arteriosclerosis : an Official Journal of the American Heart Association, Inc·C Y YangA M Gotto
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Citations

Jan 1, 1995·Journal of Clinical Laboratory Analysis·S E Hahn, D M Goldberg
Jul 26, 2012·European Biophysics Journal : EBJ·Hassan M Khachfe, David Atkinson
Jan 1, 1994·Chemistry and Physics of Lipids·C YangA M Gotto
Jan 1, 1991·Progress in Lipid Research·J BorénS O Olofsson
Jan 1, 1991·Progress in Lipid Research·D M Lee
Apr 1, 1995·Journal of the American Society for Mass Spectrometry·O BurletS J Gaskell
Jan 1, 1997·Free Radical Biology & Medicine·C Y YangC V Smith
May 11, 2010·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·Angela C RutledgeKhosrow Adeli
Dec 19, 2013·Journal of Controlled Release : Official Journal of the Controlled Release Society·Hong-Bo PangErkki Ruoslahti
Feb 7, 2012·Journal of Cellular and Molecular Medicine·Alisa G WoodsCostel C Darie
Jul 4, 1993·Biochimica Et Biophysica Acta·B LundbergD Papahadjopoulos
Oct 17, 1995·Biochimica Et Biophysica Acta·Y I MillerN Shaklai
Jan 9, 2003·Journal of Lipid Research·M Mahmood HussainPaul Dreizen
Feb 4, 2003·Journal of Lipid Research·Xingyu WangRoss W Milne
Jan 13, 2006·The Journal of Biological Chemistry·Aubrey S LedfordGregory S Shelness
Aug 28, 2003·The Journal of Biological Chemistry·Gregory S ShelnessRichard B Weinberg
Jun 4, 2014·Current Atherosclerosis Reports·Liang-Yin KeChu-Huang Chen
Apr 5, 2000·The Journal of Biological Chemistry·W L Burch, H Herscovitz
Apr 23, 2008·Annals of Medicine·Leonard J Banaszak, Wasantha K Ranatunga

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