PMID: 769681Mar 1, 1976

Isolation and characterization of tetracycline resistance proteins from Staphylococcus aureus and Escherichia coli

Antimicrobial Agents and Chemotherapy
A WojdaniD Sompolinsky

Abstract

Immunoglobulin (adsorbed resistance antiserum) reacting specifically with antigens from tetracycline (Tc)-resistant Staphylcoccus aureus or Escherichia coli was produced by adsorbing immunoglobulin against cell envelopes of resistant strains with envelope extracts from the respective isogenic susceptible strains. Adsorbed resistance antiserum against S. aureus reacted with envelope extracts from 32 Tc-resistant strains and failed to react with similar extracts from 76 Tc-susceptible strains of S. aureus. An antigen (Tc resistance antigen [TRA]) found only in Tc-resistant strains was produced by adsorbing envelope extracts from these strains with immunoglobulin against envelopes from isogenic Tc-susceptible strains. On immunodiffusion no cross-reactivity between TRAs from S. aureus and E. coli was observed. The TRAs behaved as proteins. The molecular weight of TRA from S. aureus was determined to be 32,000 and from E. coli to be 50,000. Data obtained by preliminary amino acid analysis of the TRAs are presented.

References

May 1, 1975·Journal of General Microbiology·E H Asheshov
Aug 1, 1970·Journal of General Microbiology·D SompolinskyN Abramova
Feb 27, 1974·Biochemical and Biophysical Research Communications·S B Levy, L McMurry
Sep 1, 1973·Antimicrobial Agents and Chemotherapy·D Sompolinsky, J Krausz
Dec 1, 1973·Journal of General Microbiology·I ChopraR W Lacey
Mar 1, 1967·Mutation Research·D SompolinskiI Boldur
Aug 1, 1970·Journal of General Microbiology·D SompolinskyN Abramova
Aug 1, 1974·Antimicrobial Agents and Chemotherapy·I Boldur, D Sompolinsky

Citations

Jul 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·L McMurryS B Levy
May 1, 1977·Antimicrobial Agents and Chemotherapy·V L Clark, F E Young
Sep 12, 2015·Journal of Clinical Microbiology·Jeanette W P TeoRaymond T P Lin

Related Concepts

Antigens, Bacterial
Bacterial Proteins
Cross Reactions
Antibiotic Resistance, Microbial
Alkalescens-Dispar Group
Staphylococcus aureus
Topicycline

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