Isolation and characterization of the Aspergillus oryzae gene encoding aspergillopepsin O

Gene
R M BerkaM Ward

Abstract

We have cloned and determined the nucleotide sequence of a genomic DNA segment from Aspergillus oryzae which contains pepO, the gene encoding the aspartic proteinase, aspergillopepsin O (PEPO). The organization of pepO is strikingly similar to that of pepA from A. niger var. awamori (previously called A. awamori) in that both are composed of four exons and three introns with virtually identical lengths, and the positions of the introns are exactly conserved. From the deduced amino acid (aa) sequence, it appears that PEPO, like other fungal aspartic proteinases, is synthesized as a zymogen containing a putative N-terminal prepro-region of 77 aa followed by a mature protein of 327 aa. Southern blotting experiments suggest that a single copy of pepO exists in the A. oryzae genome.

References

Jul 31, 1979·Molecular and Cellular Biochemistry·J Tang
Jun 1, 1973·Canadian Journal of Biochemistry·J E GrahamT Hofmann

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Citations

Apr 7, 1999·The Biochemical Journal·H C Ho, T H Liao
Dec 1, 1994·Journal of Medical and Veterinary Mycology : Bi-monthly Publication of the International Society for Human and Animal Mycology·U ReichardR Rüchel
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Nov 28, 2002·International Journal of Medical Microbiology : IJMM·Michel MonodOlivier Jousson
Jun 2, 2020·Journal of the Science of Food and Agriculture·Napaporn ChintagavongseHaruto Kumura
Dec 3, 1998·Applied and Environmental Microbiology·A DoumasM Monod
Aug 28, 1998·Microbiology·Peter C Farley, Patrick A Sullivan
Aug 27, 2021·Journal of Fungi·Kotaro Ito, Asahi Matsuyama

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