Isolation and characterization of the gene coding for the amidinotransferase involved in the biosynthesis of phaseolotoxin in Pseudomonas syringae pv. phaseolicola

Molecular Plant-microbe Interactions : MPMI
Gustavo Hernández-Guzmán, Ariel Alvarez-Morales

Abstract

Pseudomonas syringae pv. phaseolicola is the causal agent of the "halo blight" disease of beans. A key component in the development of the disease is a nonhost-specific toxin, Ndelta-(N'-sulphodiaminophosphinyl)-ornithyl-alanyl-homoarginine, known as phaseolotoxin. The homoarginine residue in this molecule has been suggested to be the product of L-arginine:lysine amidinotransferase activity, previously detected in extracts of P. syringae pv. phaseolicola grown under conditions of phaseolotoxin production. We report the isolation and characterization of an amidinotransferase gene (amtA) from P. syringae pv. phaseolicola coding for a polypeptide of 362 residues (41.36 kDa) and showing approximately 40% sequence similarity to L-arginine:inosamine-phosphate amidinotransferase from three species of Streptomyces spp. and 50.4% with an L-arginine:glycine amidinotransferase from human mitochondria. The cysteine, histidine, and aspartic acid residues involved in substrate binding are conserved. Furthermore, expression of the amtA and argK genes and phaseolotoxin production occurs at 18 degrees C but not at 28 degrees C. An amidinotransferase insertion mutant was obtained that lost the capacity to synthesize homoarginine and phaseolotoxi...Continue Reading

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Citations

Nov 7, 2013·International Journal of Molecular Sciences·Aleksandra Jaźwińska-KozubaStefanie M Bode-Böger
May 6, 2011·Aquatic Toxicology·Rocky ChauBrett A Neilan
Mar 28, 2013·MicrobiologyOpen·Angel Barón-SolaSoledad Sanz-Alférez
Oct 29, 2005·FEMS Microbiology Letters·Chang-Sik Oh, Steven V Beer
Nov 11, 2017·The Journal of Biological Chemistry·Sreejesh ShankerJeffrey A Lawton
Jan 25, 2018·Journal of Natural Products·Janusz J PetkowskiSara Seager

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