Isolation and characterization of the gene encoding the chloroplast-type ferredoxin component of carbazole 1,9a-dioxygenase from a putative Kordiimonas sp

Biotechnology Letters
Rintaro MaedaToshio Omori

Abstract

Carbazole (CAR)-degrading genes (carRAaCBaBb) were isolated from marine CAR-degrading isolate strain OC9 (probably Kordiimonas gwangyangensis) using shotgun cloning experiments and showed 35-65% similarity with previously reported CAR-degrading genes. In addition, a ferredoxin-like gene (carAc) was found downstream of carR, although it was not homologous with any reported ferredoxin components of the CAR 1,9a-dioxygenase (CARDO) system. The carAc-deduced amino acid sequence possessed consensus sequences for chloroplast-type iron-sulfur proteins for binding the [2Fe-2S] cluster. These car genes were arranged in the order of carAcRAaCBaBb, but carRAc and carAaCBaBb genes were the opposite orientation. Escherichia coli JM109 cells harboring pBOC91 (carAa) converted CAR to 2'-aminobiphenyl-2,3-diol at a ratio of 12%, and the transformation ratio of CAR increased from 12 to 100% when carAc was added, indicating that CarAc is the ferredoxin component of the CARDO system in strain OC9. This is the first finding of a chloroplast-type ferredoxin component in a CARDO system. Biotransformation tests with aromatic compounds revealed that the strain OC9 CarAaAc showed activity with polycyclic aromatic hydrocarbons and dioxin compounds and e...Continue Reading

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Jul 28, 2004·Bioscience, Biotechnology, and Biochemistry·Kengo InoueHideaki Nojiri
Jul 12, 2005·Journal of Molecular Biology·Hideaki NojiriToshio Omori
May 5, 2006·Applied and Environmental Microbiology·Masaaki UrataHideaki Nojiri
Dec 9, 2008·Bioscience, Biotechnology, and Biochemistry·Hiromasa UchimuraHideaki Nojiri
Feb 4, 2009·FEMS Microbiology Letters·Rintaro MaedaToshio Omori

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Citations

Jan 11, 2012·Bioscience, Biotechnology, and Biochemistry·Hideaki Nojiri
Jul 19, 2014·Bioscience, Biotechnology, and Biochemistry·Shintaro ObaHiroyuki Fuse
Dec 12, 2018·Microbiology Resource Announcements·Felipe VejaranoHideaki Nojiri

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