Isolation and characterization of the gene encoding the beta-subunit of rat follicle-stimulating hormone

DNA
S D GharibW W Chin

Abstract

Follicle-stimulating hormone (FSH) is a pituitary glycoprotein hormone that is comprised of two dissimilar subunits, alpha and beta, encoded by separate genes. We have isolated the gene encoding the beta-subunit of rat FSH by screening a rat genomic DNA-library by filter hybridization with bovine FSH-beta cDNA. Southern blot analysis of rat genomic DNA suggests that there is a single copy of the FSH-beta gene per haploid genome in the rat. The nucleotide sequence of the rat FSH-beta gene was determined and the amino acid sequence of the subunit was deduced. The gene is composed of three exons and two introns. The predicted amino acid sequence reveals that there is a 20-amino-acid signal peptide followed by a mature protein of 110 amino acid residues. Exons I, II, and III are 36, 187, and 1221 bp, respectively. Intron 1 (640 bp) interrupts the 5'-untranslated (UT) region (61 bp) and intron 2 (approximately 1 kb) interrupts the coding region between amino acid residues +34 and +35. Comparison of the amino acid sequence to those of the human and bovine FSH-beta subunits reveals 80% similarity to both species. The "CAGY" sequence, or the Cys-Ala-Gly-Tyr quartet of amino acids encoded by exon II is present in every other glycoprotei...Continue Reading

References

Dec 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·F SangerA R Coulson
Sep 16, 1976·Nature·N J Proudfoot, G G Brownlee
Feb 1, 1986·The Journal of Clinical Investigation·S D GharibW W Chin
May 1, 1988·Endocrinology·M A ShupnikW W Chin
Aug 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·K E MayoW Vale
Oct 1, 1987·Molecular and Cellular Endocrinology·J E MercerI J Clarke
Dec 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·N UenoR Guillemin
Nov 30, 1987·Biochemical and Biophysical Research Communications·S Y YingR Guillemin
Apr 29, 1988·Biochemical and Biophysical Research Communications·S ShimasakiN Ling
Jun 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·S ShimasakiR Guillemin
May 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·R G ForageJ K Findlay
Nov 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·N LingR Guillemin
Oct 1, 1985·The Journal of Endocrinology·C S Hii, S L Howell
Jan 16, 1985·Biochemical and Biophysical Research Communications·D M RobertsonD M de Kretser
Nov 27, 1985·Biochemical and Biophysical Research Communications·J RivierW Vale
Jan 1, 1972·Vitamins and Hormones·A V SchallyA Arimura
Jan 1, 1980·Methods in Enzymology·A M Maxam, W Gilbert
Jan 1, 1981·Annual Review of Biochemistry·J G Pierce, T F Parsons
Sep 25, 1982·Nucleic Acids Research·R Kole, S M Weissman
Jul 1, 1983·Analytical Biochemistry·A P Feinberg, B Vogelstein

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Citations

Nov 1, 1988·Baillière's Clinical Endocrinology and Metabolism·M E WiermanW W Chin
Dec 19, 1995·Proceedings of the National Academy of Sciences of the United States of America·U B KaiserW W Chin
Jun 11, 1992·Nucleic Acids Research·E D LampertiL Villa-Komaroff
Feb 16, 2006·General and Comparative Endocrinology·Yoko NoguchiOsamu Suzuki
May 22, 2010·Molecular and Cellular Endocrinology·Liina NagirnajaMaris Laan
Feb 27, 2015·British Poultry Science·Z HuangW Zhao
Jan 28, 2015·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Rui ZhangXiaobing Wu
Oct 31, 2017·Balsaeng'gwa saengsig·Hee Soo Kim, Sung-Ho Lee
Mar 20, 2020·Endocrinology·Luisina OngaroDaniel J Bernard

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