Isolation and characterization of the genes encoding a novel oxygenase component of angular dioxygenase from the gram-positive dibenzofuran-degrader Terrabacter sp. strain DBF63

Biochemical and Biophysical Research Communications
Kano KasugaToshio Omori

Abstract

A gram-positive bacterium Terrabacter sp. strain DBF63 is able to degrade dibenzofuran (DF) via initial dioxygenation by a novel angular dioxygenase. The dbfA1 and dbfA2 genes, which encode the large and small subunits of the dibenzofuran 4,4a-dioxygenase (DFDO), respectively, were isolated by a polymerase chain reaction-based method. DbfA1 and DbfA2 showed moderate homology to the large and small subunits of other ring-hydroxylating dioxygenases (less than 40%), respectively, and some motifs such as the Fe(II) binding site and the [2Fe-2S] cluster ligands were conserved in DbfA1. DFDO activity was confirmed in Escherichia coli cells containing the cloned dbfA1 and dbfA2 genes with the complementation of nonspecific ferredoxin and ferredoxin reductase component of E. coli. Under this condition, these cells exhibited angular dioxygenation of DF and dibenzo-p-dioxin, and monooxygenation of fluorene, but not angular dioxygenation of carbazole, xanthene, and phenoxathiin. Phylogenetic analysis revealed that DbfA1 formed a branch with recently reported large subunits of polycyclic aromatic hydrocarbon (PAH) dioxygenase from gram-positive bacteria but did not cluster with that of other angular dioxygenases, i.e., DxnA1 from Sphingomo...Continue Reading

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Citations

Apr 29, 2008·Applied and Environmental Microbiology·Hamdy A H AlyDietmar H Pieper
Dec 25, 2007·Applied and Environmental Microbiology·Luc SchulerSpiros N Agathos
Jun 6, 2006·Applied and Environmental Microbiology·Sinéad M Ní ChadhainGerben J Zylstra
Mar 10, 2004·Applied and Environmental Microbiology·Daisuke IshiyamaJulian Davies
May 5, 2006·Applied and Environmental Microbiology·Masaaki UrataHideaki Nojiri
Dec 19, 2006·Journal of Bacteriology·Masaki ShintaniHideaki Nojiri
Nov 27, 2002·Bioscience, Biotechnology, and Biochemistry·Hideaki Nojiri, Toshio Omori
May 6, 2003·Bioscience, Biotechnology, and Biochemistry·Hiroshi Habe, Toshio Omori
Mar 10, 2009·Bioscience, Biotechnology, and Biochemistry·Parichat SukdaMasao Fukuda
Dec 9, 2008·Bioscience, Biotechnology, and Biochemistry·Hiromasa UchimuraHideaki Nojiri
Dec 17, 2002·The Journal of General and Applied Microbiology·Hideaki NojiriToshio Omori
Jul 11, 2009·Biodegradation·Priyangshu Manab SarmaBanwari Lal
Jun 16, 2006·Current Microbiology·Shiwei JinYing Xu
Aug 7, 2002·Biochemical and Biophysical Research Communications·Hideaki NojiriToshio Omori
Jul 30, 2008·FEMS Microbiology Reviews·Ri-He PengQuan-Hong Yao
Apr 22, 2014·Applied and Environmental Microbiology·Chenghong WangShunpeng Li
Mar 27, 2012·Environmental Toxicology and Chemistry·Yuzo SuzukiYoshihiro Katayama
Mar 12, 2021·G3 : Genes - Genomes - Genetics·Eglantina Lopez-EcharteaOndrej Uhlik

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