Isolation and characterization of the mating-type idiomorphs from the wheat septoria leaf blotch fungus Mycosphaerella graminicola

Fungal Genetics and Biology : FG & B
C WaalwijkG H Kema

Abstract

Both mating-type loci from the wheat septoria leaf blotch pathogen Mycosphaerella graminicola have been cloned and sequenced. The MAT1-2 gene was identified by screening a genomic library from the MAT1-2 isolate IPO94269 with a heterologous probe from Tapesia yallundae. The MAT1-2 idiomorph is 2772 bp and contains a single gene encoding a putative high-mobility-group protein of 394 amino acids. The opposite idiomorph was obtained from isolate IPO323, which has the complementary mating type, by long-range PCR using primers derived from sequences flanking the MAT1-2 idiomorph. The MAT1-1 locus is 2839 bp in size and contains a single open reading frame encoding a putative alpha1-domain protein of 297 amino acids. Within the nonidiomorphic sequences, homology was found with palI, encoding a membrane receptor from Aspergillus nidulans, and a gene encoding a putative component of the anaphase-promoting complex from Schizosaccharomyces pombe and a DNA-(apurinic or apyrimidinic) lyase from S. pombe. For each of the MAT genes specific primers were designed and tested on an F1 mapping population that was generated from a cross between IPO323 and IPO94269. An absolute correlation was found between the amplified allele-specific fragments ...Continue Reading

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Citations

Jan 5, 2014·The Journal of Microbiology·Sung-Hwan YunB Gillian Turgeon
Jun 13, 2013·Archives of Microbiology·Guozhen Zhang, Yue Liang
Sep 2, 2003·Fungal Genetics and Biology : FG & B·R S BennettG C Bergstrom
Jun 20, 2012·Proceedings of the National Academy of Sciences of the United States of America·Eva Holtgrewe StukenbrockMikkel Heide Schierup
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Feb 3, 2007·Fungal Genetics and Biology : FG & B·Sarah B WareGert H J Kema
Dec 21, 2006·Fungal Genetics and Biology : FG & B·Ioannis StergiopoulosPierre J G M De Wit
Apr 26, 2015·Fungal Genetics and Biology : FG & B·A M WilsonB D Wingfield
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Nov 12, 2010·Genome Biology·Simon R EllwoodTimothy L Friesen
Nov 14, 2013·Fungal Genetics and Biology : FG & B·Wubetu BihonBrenda D Wingfield

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