Isolation and Characterization of the Novel Phages vB_VpS_BA3 and vB_VpS_CA8 for Lysing Vibrio parahaemolyticus

Frontiers in Microbiology
Meiyan YangZhiyuan Tan

Abstract

Accumulating evidence has indicated that the multiple drug resistant Vibrio parahaemolyticus may pose a serious threat to public health and economic concerns for humans globally. Here, two lytic bacteriophages, namely vB_VpS_BA3 and vB_VpS_CA8, were isolated from sewage collected in Guangzhou, China. Electron microscopy studies revealed both virions taxonomically belonged to the Siphoviridae family with icosahedral head and a long non-contractile tail. The double-stranded DNA genome of phage BA3 was composed of 58648 bp with a GC content of 46.30% while phage CA8 was 58480 bp with an average GC content of 46.42%. In total, 85 putative open reading frames (ORFs) were predicted in the phage BA3 genome while 84 were predicted in that of CA8. The ORFs were associated with phage structure, packing, host lysis, DNA metabolism, and additional functions. Furthermore, average nucleotide identity analysis, comparative genomic features and phylogenetic analysis revealed that BA3 and CA8 represented different isolates but novel members of the family, Siphoviridae. Regarding the host range of the 61 V. parahaemolyticus isolates, BA3 and CA8 had an infectivity of 8.2 and 36.1%, respectively. Furthermore, ∼100 plaque-forming units (pfu)/cell ...Continue Reading

References

Dec 15, 2000·Journal of Bacteriology·F TétartH M Krisch
Mar 15, 2001·Diagnostic Microbiology and Infectious Disease·M LesmanaB A Oyofo
Oct 24, 2003·Current Opinion in Microbiology·Roger W Hendrix
Sep 9, 2004·Microbiology and Molecular Biology Reviews : MMBR·Fabiano L ThompsonJean Swings
Oct 27, 2004·Infection and Immunity·Kwon-Sam ParkTakeshi Honda
Jun 25, 2005·Bioinformatics·Tim J CarverJulian Parkhill
Jun 27, 2007·Proceedings of the National Academy of Sciences of the United States of America·Timothy K Lu, James J Collins
Oct 1, 2008·Current Opinion in Microbiology·Graham F Hatfull
Dec 11, 2008·Methods in Molecular Biology·Andrew M KropinskiRoger P Johnson
Dec 11, 2008·Methods in Molecular Biology·Elizabeth Kutter
Mar 4, 2009·Proceedings of the National Academy of Sciences of the United States of America·Timothy K Lu, James J Collins
Feb 1, 2011·Bioinformatics·Mitchell J SullivanScott A Beatson
Apr 18, 2012·Journal of Computational Biology : a Journal of Computational Molecular Cell Biology·Anton BankevichPavel A Pevzner
Jun 26, 2013·International Journal of Antimicrobial Agents·Donatella OttavianiC Pazzani
Mar 20, 2014·Bioinformatics·Torsten Seemann
Aug 15, 2015·BioMed Research International·Anna KrasowskaMarcin Łukaszewicz
Oct 5, 2016·Biologicals : Journal of the International Association of Biological Standardization·Nattan Stalin, Pappu Srinivasan
Oct 19, 2017·Nucleic Acids Research·Elliot J LefkowitzDonald B Smith
Nov 15, 2017·Methods in Molecular Biology·Xianglilan ZhangYigang Tong
May 4, 2018·Molecular Biology and Evolution·Sudhir KumarKoichiro Tamura
Aug 6, 2018·Research in Microbiology·Hin-Chung WongWei-Hau Chang
Aug 15, 2018·Nature Reviews. Microbiology·Franklin L NobregaStan J J Brouns
Nov 7, 2018·Nucleic Acids Research·Alex L MitchellRobert D Finn
Jan 13, 2019·Advances in Virus Research·Jesse Cahill, Ry Young
Mar 1, 2019·Viruses·Cynthia Maria ChibaniHeiko Liesegang

❮ Previous
Next ❯

Citations

Jan 21, 2021·Pharmaceuticals·Nejat DüzgüneşMetin Yildirim
Feb 25, 2021·The Journal of Microbiology·Kira Moon, Jang-Cheon Cho
Jul 9, 2021·Frontiers in Microbiology·Yuting ShangJumei Zhang

❮ Previous
Next ❯

Datasets Mentioned

BETA
MN175679
MN102376
MK892600.1
KM378617

Methods Mentioned

BETA
PCR
electrophoresis
Infection
environmental stresses
phage therapy

Software Mentioned

Prokka
BLAST
InterPro
pyani package
MaxQuant
BLASTp
MEGA X
Artemis Comparison Tool ( ACT )
BLASTN
Easyfig

Related Concepts

Related Feeds

Bacteriophage: Phage Therapy

Phage therapy uses bacterial viruses (bacteriophages) to treat bacterial infections and is widely being recognized as an alternative to antibiotics. Here is the latest research.

Related Papers

Biochimica Et Biophysica Acta
Min-Syuan HuangMingli Hsieh
Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine
Tze-Kai WangMingli Hsieh
© 2021 Meta ULC. All rights reserved