PMID: 6965795Jan 1, 1980Paper

Isolation and characterization of the pigment-protein complexes of Rhodopseudomonas sphaeroides by lithium dodecyl sulfate/polyacrylamide gel electrophoresis

Proceedings of the National Academy of Sciences of the United States of America
R M BroglieR K Clayton

Abstract

When purified photosynthetic membranes from Rhodopseudomonas sphaeroides were treated with lithium dodecyl sulfate and subjected to polyacrylamide gel electrophoresis at 4 degrees C, up to 11 pigment-protein complexes were resolved. Absorption spectra revealed that the smallest complex contained reaction center pigments and the others contained the antenna components B850 and B875 in various proportions. Of these antenna complexes, the largest was almost entirely B850 and the smallest contained only B875. After solubilization at 100 degrees C and electrophoresis on polyacrylamide gradient gels, the B850 complex gave rise to two polypeptide components migrating with apparent Mr of 10,000 and 8000, whereas with the B875 complex, two components were observed with apparent Mr of 12,000 and 8000. The reaction center complex gave rise to only the 24 and 21 kilodalton polypeptide subunits. Fluorescence emission spectra showed maxima at 872 and 902 nm for B850 and B875, respectively. Analyses of bacteriochlorophyll a and carotenoids indicated that, in the B875 complex, two molecules of each of these pigments are associated with the two polypeptides. The associations of B850 and B875 in large and small complexes obtained by lithium dode...Continue Reading

References

Jan 1, 1978·Annual Review of Biochemistry·R E Blankenship, W W Parson
Dec 14, 1972·Biochimica Et Biophysica Acta·R K Clayton, B J Clayton
Feb 1, 1972·Photochemistry and Photobiology·J Aagaard, W R Sistrom
Dec 1, 1968·Proceedings of the National Academy of Sciences of the United States of America·K L ZankelR K Clayton
Jan 1, 1969·Photochemistry and Photobiology·K L Zankel, R K Clayton

❮ Previous
Next ❯

Citations

Mar 1, 1990·Photosynthesis Research·J N Sturgis, R A Niederman
Jan 1, 1988·Photosynthesis Research·T J DonohueS Kaplan
Jan 1, 1988·Comparative Biochemistry and Physiology. C, Comparative Pharmacology and Toxicology·M E HarrisD P Selivonchick
Oct 20, 2005·Journal of Bioscience and Bioengineering·Toshihiko KondoJun Miyake
Mar 10, 2010·Proceedings of the National Academy of Sciences of the United States of America·Camille Mascle-AllemandJames N Sturgis
Sep 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·R K Clayton, B J Clayton
Oct 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·A E BrownC J Arntzen
May 14, 2009·The Journal of Biological Chemistry·Juliette Jouhet, John C Gray
Dec 1, 1994·Photochemistry and Photobiology·J D Olsen, C N Hunter
Dec 1, 2004·The Journal of Cell Biology·Juliette JouhetMaryse A Block
Jul 29, 2015·Photosynthesis Research·Erica L-W MajumderRobert E Blankenship
Nov 1, 2015·Biochimica Et Biophysica Acta·Robert A Niederman
Nov 25, 2005·Photochemical & Photobiological Sciences : Official Journal of the European Photochemistry Association and the European Society for Photobiology·C Neil HunterRobert A Niederman
May 10, 2006·The Journal of Biological Chemistry·Jing ZhengMaryAnn Cheatham
Apr 4, 2020·The Journal of Biological Chemistry·Donald A BryantMartin J Warren
Jul 1, 1984·Hoppe-Seyler's Zeitschrift für physiologische Chemie·R TheilerH Zuber
Jan 1, 1983·Journal of Cellular Biochemistry·J P ThornberR E Seftor

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.