Isolation and characterization of the promoter sequence of a cassava gene coding for Pt2L4, a glutamic acid-rich protein differentially expressed in storage roots

Genetics and Molecular Research : GMR
Cláudia Regina Batista de SouzaLuiz Joaquim Castelo Branco Carvalho

Abstract

Cassava is one of the most important tropical food crops for more than 600 million people worldwide. Transgenic technologies can be useful for increasing its nutritional value and its resistance to viral diseases and insect pests. However, tissue-specific promoters that guarantee correct expression of transgenes would be necessary. We used inverse polymerase chain reaction to isolate a promoter sequence of the Mec1 gene coding for Pt2L4, a glutamic acid-rich protein differentially expressed in cassava storage roots. In silico analysis revealed putative cis-acting regulatory elements within this promoter sequence, including root-specific elements that may be required for its expression in vascular tissues. Transient expression experiments showed that the Mec1 promoter is functional, since this sequence was able to drive GUS expression in bean embryonic axes. Results from our computational analysis can serve as a guide for functional experiments to identify regions with tissue-specific Mec1 promoter activity. The DNA sequence that we identified is a new promoter that could be a candidate for genetic engineering of cassava roots.

Citations

Feb 15, 2013·Genetics and Molecular Research : GMR·F XuS Y Cheng
Jan 21, 2016·Plant Signaling & Behavior·Valerie Bowrin, Fedora Sutton
May 14, 2011·Journal of Integrative Plant Biology·Jia LiuPeng Zhang
Mar 26, 2011·The Plant Journal : for Cell and Molecular Biology·Judith OwitiHervé Vanderschuren
Jan 25, 2017·The New Phytologist·Mark C WilsonRebecca S Bart
Jan 15, 2014·Molecular Biology Reports·Ailton Borges Santa BrígidaCláudia Regina Batista de Souza

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