PMID: 139921Apr 1, 1977

Isolation and characterization of the surface membranes of fast and slow mammalian skeletal muscle

Biochimica Et Biophysica Acta
P B Smith, S H Appel

Abstract

Fast (extensor digitorum longus) and slow (soleus) rat skeletal muscles served as the source for isolation and biochemical comparison of two distinct surface membrane fractions with properties of the sarcolemma and transverse tubular system. Enriched sarcolemmal membrane from soleus demonstrated a lighter density after sucrose density centrifugation. Sialic acid content was 1.5-fold higher in soleus (62 nmol/mg) than extensor (40 nmol/mg). The specific activity of (Na+ + K+ + Mg2+)-ATPase was similar (1.40 and 1.65 micronmol Pi/mg per 5 min) with the soleus enzyme displaying a (1) greater resistance to inhibition by ouabain, and (2) broader ionic ratio (Na+/K+) requirement than extensor enzyme. The polypeptide and phospholipid composition showed no major differences between the two muscle types. The second surface membrane fraction, tentatively identified as transverse tubule, differed in membrane composition. The major polypeptide of extensor was of 95 000 molecular weight whereas for soleus a Mr=28 000 species was dominant. Total phospholipid content of soleus was 1.5-fold greater than extensor due mostly to increased levels of phosphatidylcholine and phosphatidylethanolamine. Endogenous membrane protein kinase for the 28 000...Continue Reading

References

Aug 13, 1971·Biochimica Et Biophysica Acta·G Meissner, S Fleischer
Jan 1, 1970·Experimental Neurology·E X Albuquerque, R J McIsaac
Sep 1, 1974·The Journal of Clinical Investigation·D S Campion
Feb 10, 1967·Annals of the New York Academy of Sciences·W C Bowman, C Raper
Jan 1, 1969·Biochimica Et Biophysica Acta·K PuroJ K Huttunen

Citations

Apr 1, 1989·Journal of Bioenergetics and Biomembranes·R A Sabbadini, A S Dahms
Jan 1, 1980·The International Journal of Biochemistry·F I SevillanoJ A Cabezas
Jan 1, 1980·Progress in Biophysics and Molecular Biology·P V Sulakhe, P J St Louis
May 1, 1978·Experimental Neurology·P B SmithS H Appel
Mar 10, 1980·FEBS Letters·E N Olson, P B Smith
Jan 1, 1981·Acta Neuropathologica·D L SchotlandY Wakayama
Jul 12, 2002·American Journal of Physiology. Endocrinology and Metabolism·Bernd WalzelTheo Wallimann
Feb 19, 2005·Doklady Biological Sciences : Proceedings of the Academy of Sciences of the USSR, Biological Sciences Sections·I V KovyazinaE E Nikolsky

Related Concepts

DNA-dependent ATPase
Cell Fractionation
Plasma Membrane
Centrifugation, Density Gradient
Enzyme Activation
Magnesium
Tissue Membrane
Muscle
Polypeptides
Potassium

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