PMID: 139921Apr 1, 1977

Isolation and characterization of the surface membranes of fast and slow mammalian skeletal muscle

Biochimica Et Biophysica Acta
P B Smith, S H Appel


Fast (extensor digitorum longus) and slow (soleus) rat skeletal muscles served as the source for isolation and biochemical comparison of two distinct surface membrane fractions with properties of the sarcolemma and transverse tubular system. Enriched sarcolemmal membrane from soleus demonstrated a lighter density after sucrose density centrifugation. Sialic acid content was 1.5-fold higher in soleus (62 nmol/mg) than extensor (40 nmol/mg). The specific activity of (Na+ + K+ + Mg2+)-ATPase was similar (1.40 and 1.65 micronmol Pi/mg per 5 min) with the soleus enzyme displaying a (1) greater resistance to inhibition by ouabain, and (2) broader ionic ratio (Na+/K+) requirement than extensor enzyme. The polypeptide and phospholipid composition showed no major differences between the two muscle types. The second surface membrane fraction, tentatively identified as transverse tubule, differed in membrane composition. The major polypeptide of extensor was of 95 000 molecular weight whereas for soleus a Mr=28 000 species was dominant. Total phospholipid content of soleus was 1.5-fold greater than extensor due mostly to increased levels of phosphatidylcholine and phosphatidylethanolamine. Endogenous membrane protein kinase for the 28 000...Continue Reading


Aug 13, 1971·Biochimica Et Biophysica Acta·G Meissner, S Fleischer
Jan 1, 1970·Experimental Neurology·E X Albuquerque, R J McIsaac
Sep 1, 1974·The Journal of Clinical Investigation·D S Campion
Feb 10, 1967·Annals of the New York Academy of Sciences·W C Bowman, C Raper
Jan 1, 1969·Biochimica Et Biophysica Acta·K PuroJ K Huttunen


Apr 1, 1989·Journal of Bioenergetics and Biomembranes·R A Sabbadini, A S Dahms
Jan 1, 1980·The International Journal of Biochemistry·F I SevillanoJ A Cabezas
Jan 1, 1980·Progress in Biophysics and Molecular Biology·P V Sulakhe, P J St Louis
May 1, 1978·Experimental Neurology·P B SmithS H Appel
Mar 10, 1980·FEBS Letters·E N Olson, P B Smith
Jan 1, 1981·Acta Neuropathologica·D L SchotlandY Wakayama
Jul 12, 2002·American Journal of Physiology. Endocrinology and Metabolism·Bernd WalzelTheo Wallimann
Feb 19, 2005·Doklady Biological Sciences : Proceedings of the Academy of Sciences of the USSR, Biological Sciences Sections·I V KovyazinaE E Nikolsky

Related Concepts

DNA-dependent ATPase
Cell Fractionation
Plasma Membrane
Centrifugation, Density Gradient
Enzyme Activation
Tissue Membrane

Trending Feeds


Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Alzheimer's Disease: MS4A

Variants within the membrane-spanning 4-domains subfamily A (MS4A) gene cluster have recently been implicated in Alzheimer's disease in genome-wide association studies. Here is the latest research on Alzheimer's disease and MS4A.

Pediculosis pubis

Pediculosis pubis is a disease caused by a parasitic insect known as Pthirus pubis, which infests human pubic hair, as well as other areas with hair including eye lashes. Here is the latest research.

Rh Isoimmunization

Rh isoimmunization is a potentially preventable condition that occasionally is associated with significant perinatal morbidity or mortality. Discover the latest research on Rh Isoimmunization here.

Genetic Screens in iPSC-derived Brain Cells

Genetic screening is a critical tool that can be employed to define and understand gene function and interaction. This feed focuses on genetic screens conducted using induced pluripotent stem cell (iPSC)-derived brain cells. It also follows CRISPR-Cas9 approaches to generating genetic mutants as a means of understanding the effect of genetics on phenotype.

Enzyme Evolution

This feed focuses on molecular models of enzyme evolution and new approaches (such as adaptive laboratory evolution) to metabolic engineering of microorganisms. Here is the latest research.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Pharmacology of Proteinopathies

This feed focuses on the pharmacology of proteinopathies - diseases in which proteins abnormally aggregate (i.e. Alzheimer’s, Parkinson’s, etc.). Discover the latest research in this field with this feed.

Alignment-free Sequence Analysis Tools

Alignment-free sequence analyses have been applied to problems ranging from whole-genome phylogeny to the classification of protein families, identification of horizontally transferred genes, and detection of recombined sequences. Here is the latest research.

Related Papers

Neurological Sciences : Official Journal of the Italian Neurological Society and of the Italian Society of Clinical Neurophysiology
M MirabellaS Servidei
Proceedings of the National Academy of Sciences of the United States of America
R L BarchiM Wong
© 2021 Meta ULC. All rights reserved