Isolation and characterization of the UDP-glucose 4'-epimerase-encoding gene, galE, from Brucella abortus 2308

Gene
A J Scupham, E W Triplett

Abstract

The UDP-glucose 4'-epimerase-encoding gene, galE or exoB, was isolated from Brucella abortus 2308 by complementation of an exoB mutant of Sinorhizobium meliloti. Confirmation of the identity was done by constructing an in-frame deletion of 660 bp with galE of the B. abortus genome by marker exchange. The resulting galE mutant lacked UDP-glucose 4'-epimerase activity. This activity was restored by in trans complementation with the intact gene. The B. abortus gal E mutant is not altered in colony morphology compared to wt 2308. The lack of UDP-glucose 4'-epimerase activity in the mutant and PCR analysis strongly suggest that only one copy of galE exists in B. abortus 2308. The galE sequence of B. abortus 2308 is more similar to galE from other animal-inhabiting bacteria than it is to exoB from the Sinorhizobium legume symbionts. We propose that galE in B. abortus evolved by lateral transfer from other animal-inhabiting bacteria rather than from a common ancestor of Brucella and Sinorhizobium.

References

Nov 11, 1994·Nucleic Acids Research·M BorodovskyE V Koonin
Feb 1, 1994·Trends in Genetics : TIG·J A Leigh, G C Walker
May 10, 2007·Molecular Biology and Evolution·Koichiro TamuraSudhir Kumar

❮ Previous
Next ❯

Citations

Nov 5, 2002·Veterinary Microbiology·Richard C EssenbergIan Paulsen
May 18, 2012·Bioconjugate Chemistry·Mingsheng ChenJian Sun
Mar 21, 2001·Biotechnology & Genetic Engineering Reviews·M G GilliganP F Searle
Jul 27, 2005·Molecular Plant-microbe Interactions : MPMI·John Beck JensenT V Bhuvaneswari
Oct 23, 2004·Molecular Plant-microbe Interactions : MPMI·A Leonardo IniguezEric W Triplett
Aug 30, 2002·Applied and Environmental Microbiology·Alexandra J ScuphamEric W Triplett
Nov 22, 2005·Infection and Immunity·Patrick S G ChainEmilio Garcia
Jul 18, 2014·Veterinary Research·Pedro Soler-LlorénsIgnacio López-Goñi
Jan 30, 2018·Frontiers in Microbiology·Raquel Conde-ÁlvarezMaite Iriarte
Jun 13, 2017·Critical Reviews in Microbiology·T BarbierJ J Letesson
Oct 16, 2018·Frontiers in Microbiology·Miriam Salvador-BescósRaquel Conde-Álvarez
Feb 2, 2021·Frontiers in Microbiology·Leticia Lázaro-AntónAmaia Zúñiga-Ripa

❮ Previous
Next ❯

Related Concepts

Related Feeds

Brucellosis (ASM)

Brucellosis is a bacterial infection caused by members of the genus brucella and remains one of the world's major zoonotic diseases. Discover the latest research on Brucellosis here.

Brucellosis

Brucellosis is a bacterial infection caused by members of the genus brucella and remains one of the world's major zoonotic diseases. Discover the latest research on Brucellosis here.