Isolation and Characterization of the Acinetobactin and Baumannoferrin Siderophores Produced by Acinetobacter baumannii

Methods in Molecular Biology
William F Penwell, Luis A Actis

Abstract

Siderophores are high-affinity iron chelators produced and used by bacteria to prosper under iron-limiting conditions they normally encounter in the environment and hosts. In this chapter, we describe the isolation and purification of the siderophores acinetobactin and baumannoferrin produced by the bacterial pathogen Acinetobacter baumannii using XAD-7 batch adsorption and high-pressure liquid chromatography (HPLC). We also describe chemical tests and biological assays used to detect the presence of catechol and hydroxamate siderophores in culture supernatants, XAD-7 extracts, and HPLC fractions.

Citations

May 10, 2020·Biomolecules·Maria Soledad RamirezMarcelo E Tolmasky
Feb 23, 2020·International Microbiology : the Official Journal of the Spanish Society for Microbiology·Lei ZhaoShuang Kong
Oct 17, 2019·ACS Infectious Diseases·Zhijuan Hu, Wenjun Zhang

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