Isolation and characterization of three maize aquaporin genes, ZmNIP2;1, ZmNIP2;4 and ZmTIP4;4 involved in urea transport

BMB Reports
Riliang GuLixing Yuan

Abstract

Urea-based nitrogen fertilizer was widely utilized in maize production, but transporters involved in urea uptake, translocation and cellular homeostasis have not been identified. Here, we isolated three maize aquapoin genes, ZmNIP2;1, ZmNIP2;4 and ZmTIP4;4, from a cDNA library by heterogeneous complementation of a urea uptake-defective yeast. ZmNIP2;1 and ZmNIP2;4 belonged to the nodulin 26-like intrinsic proteins (NIPs) localized at plasma membrane, and ZmTIP4;4 belonged to the tonoplast intrinsic protein (TIPs) at vacuolar membrane. Quantitative RT-PCR revealed that ZmNIP2;1 was expressed constitutively in various organs while ZmNIP2;4 and ZmTIP4;4 transcripts were abundant in reproductive organs and roots. Expression of ZmTIP4;4 was significantly increased in roots and expanded leaves under nitrogen starvation, while those of ZmNIP2;1 and ZmNIP2;4 remained unaffected. Functions of maize aquapoin genes in urea transport together with their distinct expression manners suggested that they might play diverse roles on urea uptake and translocation, or equilibrating urea concentration across tonoplast.

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Citations

Nov 29, 2014·Critical Reviews in Biotechnology·Ashish Kumar SrivastavaLam-Son Phan Tran
Sep 5, 2015·Plant Signaling & Behavior·Roberto PintonLaura Zanin
Dec 3, 2013·The Journal of Membrane Biology·Juliana Perez Di GiorgioNicolás Daniel Ayub
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Jan 19, 2018·International Journal of Molecular Sciences·Limin GaoQirong Shen
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Sep 11, 2020·Physiologia Plantarum·Gloria BarzanaMicaela Carvajal
Dec 7, 2020·Physiologia Plantarum·Marcel P Beier, Soichi Kojima
Dec 19, 2020·Scientific Reports·Alvaro Lopez-ZaplanaGloria Bárzana
Mar 29, 2021·Plant Physiology and Biochemistry : PPB·Sara BuosoLaura Zanin
Jul 25, 2021·International Journal of Molecular Sciences·Jean-Stéphane VenissePhilippe Label
Jul 11, 2021·Physiologia Plantarum·Sara BuosoLaura Zanin

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