Isolation and characterization of Tri3, a gene encoding 15-O-acetyltransferase from Fusarium sporotrichioides.

Applied and Environmental Microbiology
S P McCormickA E Desjardins

Abstract

An acetyltransferase gene (Tri3) was isolated from Fusarium sporotrichioides by complementation of a previously identified Tri3- mutant and shown to be closely linked to three other trichothecene biosynthetic pathway genes. Comparison of the Tri3 sequence with its cDNA revealed the presence of four introns. The Tri3 cDNA contains a 1,539-bp open reading frame that encodes a protein with a molecular mass of 57,418 Da. Regulation of Tri3 transcription in liquid cultures appeared identical to that of other trichothecene pathway genes. Disruption of the Tri3 gene resulted in the accumulation of deacetylated calonectrins rather than T-2 toxin. The results of whole-cell feeding experiments with Tri3- strains suggested that 15-O-acetylation is blocked. Cell-free feeding experiments confirmed that Tri3- strains are able to acetylate a trichothecene C-3 hydroxyl group but are unable to acetylate a trichothecene C-15 hydroxyl group. Our results show that Tri3 encodes an acetyltransferase that converts 15-decalonectrin to calonectrin.

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Citations

Apr 27, 2002·Applied and Environmental Microbiology·Theresa LeeYin-Won Lee
Apr 7, 2004·Applied and Environmental Microbiology·S P McCormickA E Desjardins
Apr 11, 2006·Canadian Journal of Microbiology·Susan P McCormickRobert H Proctor
Jul 16, 2005·Food Additives and Contaminants·A QuartaA Visconti
Nov 10, 2011·Toxins·Susan P McCormickNancy J Alexander
Apr 25, 2020·Applied Microbiology and Biotechnology·R H ProctorS Gutiérrez
Jul 1, 1999·FEMS Microbiology Letters·M J Sweeney, A D Dobson
May 2, 2000·Applied and Environmental Microbiology·L ChenT M Hohn
Oct 9, 2003·Applied and Environmental Microbiology·Andrew W PeplowMarian N Beremand
Sep 9, 2008·Bioscience, Biotechnology, and Biochemistry·Takeshi TokaiMakoto Kimura
Sep 11, 2007·Bioscience, Biotechnology, and Biochemistry·Makoto KimuraMakoto Fujimura
Nov 3, 2005·Proceedings of the National Academy of Sciences of the United States of America·Carin JansenFrank J Maier
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