PMID: 15354353Sep 10, 2004Paper

Isolation and characterization of two cDNAs encoding translation initiation factor 1A from rice(Oryza sativa L.)

DNA Sequence : the Journal of DNA Sequencing and Mapping
Ji HuangHongsheng Zhang

Abstract

The eukaryotic initiation factor 1A(eIF1A) is essential for transferring of the initiator Met-tRNA to 40S ribosomal subunits to form the 40S pre-initiation complex. In present study, we describe the cloning and characterization of two eIF1A genes from rice, which were designated as Oryza sativa eukaryotic initiation factor 1A genes OseIF1A-1, OseIF1A-2, respectively. Both rice elF1As shared high identities in amino acids with eIF1A proteins from other eukaryotes. The mRNA expression analysis revealed that OseIF1A-2 mRNA was much more accumulated than OseIF1A-1 in all tissues but each gene is expressed in root, stem, leaf and flowering spike in high and nearly equal level, and in immature spike in lower level. These results, together with their different location in unrooted phylogenetic tree inferred from amino acid sequences of all known eIF1As, suggested that there are two types of eIF1A genes with different function or different regulation in rice.

References

Apr 1, 1990·Plant Molecular Biology·K S ReeceR Wu
Sep 29, 1995·The Journal of Biological Chemistry·C L WeiJ W Hershey
Feb 6, 2003·Proceedings of the National Academy of Sciences of the United States of America·Assen MarintchevGerhard Wagner
Apr 26, 2003·The Plant Journal : for Cell and Molecular Biology·Antonio RausellRoc Ros

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Citations

Sep 3, 2009·Journal of Integrative Plant Biology·Wen-Ying LiuHong-Sheng Zhang

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