PMID: 9171387Jun 1, 1997Paper

Isolation and characterization of two genes, waaC (rfaC) and waaF (rfaF), involved in Pseudomonas aeruginosa serotype O5 inner-core biosynthesis

Journal of Bacteriology
T R de Kievit, J S Lam

Abstract

Recent studies have provided evidence to implicate involvement of the core oligosaccharide region of Pseudomonas aeruginosa lipopolysaccharide (LPS) in adherence to host tissues. To better understand the role played by LPS in the virulence of this organism, the aim of the present study was to clone and characterize genes involved in core biosynthesis. The inner-core regions of P. aeruginosa and Salmonella enterica serovar Typhimurium are structurally very similar; both contain two main chain residues of heptose linked to lipid A-Kdo2 (Kdo is 3-deoxy-D-manno-octulosonic acid). By electrotransforming a P. aeruginosa PAO1 library into Salmonella waaC and waaF (formerly known as rfaC and rfaF, respectively) mutants, we were able to isolate the homologous heptosyltransferase I and II genes of P. aeruginosa. Two plasmids, pCOREc1 and pCOREc2, which restored smooth LPS production in the waaC mutant, were isolated. Similarly, plasmid pCOREf1 was able to complement the Salmonella waaF mutant. Sequence analysis of the DNA insert of pCOREc2 revealed one open reading frame (ORF) which could code for a protein of 39.8 kDa. The amino acid sequence of the deduced protein exhibited 53% identity with the sequence of the WaaC protein of S. enter...Continue Reading

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Citations

Aug 28, 2009·Innate Immunity·Jerry D KingJoseph S Lam
Jul 18, 2020·Applied Microbiology and Biotechnology·Sandrine M SohRobert J Mitchell
Oct 18, 2011·Biochemistry. Biokhimii︠a︡·D Kocíncová, J S Lam
Mar 25, 2004·Infection and Immunity·Margaret I KanipesPatricia Guerry
Jan 23, 1999·Infection and Immunity·B A BauerE J Hansen
Jul 27, 1999·Journal of Bacteriology·L MindichD Hoogstraten
Sep 8, 1999·Microbiology and Molecular Biology Reviews : MMBR·H L RocchettaJ S Lam

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