Isolation and characterization of two IgE-reactive proteins from Azadirachta indica pollen

Molecular and Cellular Biochemistry
P R Karmakar, B P Chatterjee

Abstract

Two allergenically active components present in the Azadirachta indica whole pollen extract have been isolated by sequential ammonium sulfate precipitation (0-90%), DEAE-Sephadex A-50 ion-exchange chromatography followed by gel filtration through Sephadex G-200. The allergenicity of fractionated materials has been tested by skin prick test and ELISA inhibition which reveal that AIaI and AIaIVb are the major allergens. Immunoblot confirms the IgE-binding activity of the proteins. Although both fractions are found to be homogeneous by SDS-PAGE, isoelectric focusing produces more than one isoelectric point in AIaI (pI = 3.15, 3.3 and 3.5) and AIaIVb (pI = 6.0 and 6.2). Amino acid analyses of the two allergens, the effect of pH on them and cross-reactivity between them have been discussed.

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Citations

Aug 22, 2013·Environmental Monitoring and Assessment·Mir Musaraf HussainKashinath Bhattacharya
May 16, 1998·Annals of Allergy, Asthma & Immunology : Official Publication of the American College of Allergy, Asthma, & Immunology·P ChakrabortyS Chanda
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Jul 21, 2004·Journal of Ethnopharmacology·Sara J BoekeIvonne M C M Rietjens
Feb 6, 2007·International Immunopharmacology·Koustav SarkarRathindranath Baral

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