Isolation and characterization of type IV group B Streptococcus capsular polysaccharide.

Infection and Immunity
M R WesselsD L Kasper

Abstract

An antigenically distinct serotype, type IV, has recently been added to the recognized serotypes of group B streptococci (GBS). We isolated and purified the capsular polysaccharide antigen from a prototype type IV GBS strain. The type IV capsular polysaccharide formed a precipitin line with rabbit antiserum to type IV GBS organisms but not with antiserum to organisms of GBS serotype Ia, Ib, II, or III. Enzyme-linked immunosorbent assay inhibition experiments showed no cross-reaction between type IV antiserum and other GBS serotypes. Capsular polysaccharide released from the bacterial cells with mutanolysin and that isolated from the culture supernatant had similar elution profiles on Sepharose CL-6B, with a Kav of 0.30 and an estimated Mr of 200,000. The purified type IV polysaccharide was found to contain galactose, glucose, N-acetylglucosamine, and N-acetylneuraminic acid (sialic acid) as exclusive sugars. The polysaccharide contained 23% (by weight) sialic acid and galactose, glucose, and N-acetylglucosamine in a relative ratio of (1):1.10:0.55. These results are compatible with a repeating structure of six monosaccharide residues containing galactose, glucose, N-acetylglucosamine, and sialic acid in a molar ratio of 2:2:1:1...Continue Reading

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Citations

Jul 1, 1992·Zentralblatt für Veterinärmedizin. Reihe B. Journal of veterinary medicine. Series B·I W Wibawan, C Lämmler
Sep 23, 2008·Expert Reviews in Molecular Medicine·Heather C MaiseyVictor Nizet
Jan 1, 1994·Microbiology and Immunology·F QadriM J Albert
Oct 24, 2006·Infection and Immunity·Andrea HamiltonAmanda L Jones
Dec 22, 2004·The Journal of Immunology : Official Journal of the American Association of Immunologists·Youko AoyagiJohn F Bohnsack
Jun 16, 2017·Journal of Clinical Microbiology·Areej AlhhazmiGregory J Tyrrell
Jan 4, 2001·Microbes and Infection·B Spellerberg
Mar 16, 2019·Microbiology Spectrum·Lawrence C Paoletti, Dennis L Kasper
Jun 3, 2021·Pathogens·Ze Zhang
Apr 1, 1993·The Journal of Pediatrics·M A Rench, C J Baker

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