Isolation and characterization of undersulphated chondroitin-4-sulphate from normal human plasma.

Biochimica Et Biophysica Acta
M JuvaniO Wegelius

Abstract

The present study was undertaken in order to characterize further the glycosaminoglycans of normal human plasma. Coagulation factor IX concentrate prepared from undiluted plasma by DEAE-Sephadex chromatography was used as the starting material. The concentrate was subjected to proteolytic treatment with papain and pronase, deproteinised with trichloroacetic acid, dialysed and passed through an AG 1 X 2 anion-exchange column. Glycosaminoglycans were eluted stepwise from the column with NaC1. The sole glycosaminoglycan obtained was an undersulphated chondroitin-4-sulphate which was identified by chemical analyses, digestibility with testicular hyaluronidase, electrophoretic behaviour and infrared spectrum. Gel-exclusion chromatography indicated a molecular weight of 17 000 for the compound. The undersulphated chondroitin-4-sulphate was calculated to represent at least 80% of the macromolecular glycosaminoglycans present in normal human plasma and to occur in a concentration of approx. 3 mg hexuronate per 1 of plasma.

References

Jun 1, 1966·Proceedings of the Society for Experimental Biology and Medicine·C Friman, R Brunish
Feb 1, 1969·The Journal of Clinical Investigation·A CalatroniN Di Ferrante
Jun 1, 1971·Analytical Biochemistry·T T Terho, K Hartiala
Jun 1, 1972·Biochemical Medicine·K MurataT Okuyama
Sep 1, 1957·The Journal of Clinical Investigation·A J BOLLETW F SIMPSON
May 1, 1958·The Journal of Clinical Investigation·G P KERBY
Dec 1, 1962·Archives of Biochemistry and Biophysics·D LAGUNOFF, G WARREN

❮ Previous
Next ❯

Citations

Jan 1, 1992·Rheumatology International·A BaiciF J Wagenhäuser
Jun 19, 2008·Analytical and Bioanalytical Chemistry·Angelo ZinelluCiriaco Carru
Jan 1, 1991·Comparative Biochemistry and Physiology. B, Comparative Biochemistry·A M FerlazzoA Calatroni
Apr 4, 1998·Clinica Chimica Acta; International Journal of Clinical Chemistry·L CalabròA Calatroni
Nov 1, 1985·The Journal of Clinical Investigation·I Staprans, J M Felts
Dec 17, 2008·Connective Tissue Research·Alberto CalatroniGiuseppe M Campo
Jun 7, 2005·Clinica Chimica Acta; International Journal of Clinical Chemistry·Nicola Volpi, Francesca Maccari
Jan 1, 1980·CRC Critical Reviews in Biochemistry·B Chakrabarti, J W Park
Jan 1, 1977·Scandinavian Journal of Rheumatology·C Friman, M Juvani
Jun 1, 1981·Scandinavian Journal of Clinical and Laboratory Investigation·J H Poulsen
Jul 23, 2013·Journal of Pharmaceutical and Biomedical Analysis·Nicola VolpiGiovanni V Coppa
Nov 1, 1987·Journal of Periodontal Research·P M Bartold
Feb 15, 1977·Clinica Chimica Acta; International Journal of Clinical Chemistry·K Murata, Y Horiuchi

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.