Isolation and characterization of vasohibin-2 as a homologue of VEGF-inducible endothelium-derived angiogenesis inhibitor vasohibin

Arteriosclerosis, Thrombosis, and Vascular Biology
Takumi ShibuyaYasufumi Sato

Abstract

We recently isolated vasohibin, a novel vascular endothelial growth factor (VEGF)-inducible endothelium-derived angiogenesis inhibitor. Our aim is to find DNA sequences homologous to vasohibin and determine their expression profile. By the search of DNA sequences in the database, we found one homologous gene and designated it vasohibin-2. Overall amino acid sequence homology between the prototype vasohibin (vasohibin-1) and vasohibin-2 was >50%. Vasohibin-2 exhibited antiangiogenic activity. Vasohibin-2 expression in cultured endothelial cells was low and not inducible by the stimulation that induced vasohibin-1. However, the immunohistochemical analysis revealed that vasohibin-1 and -2 were diffusely expressed in endothelial cells in embryonic organs during mid-gestation. After that time point, vasohibin-1 and -2 became faint, but persisted to a certain extent in arterial endothelial cells from late gestation to neonate. Expression of vasohibin-1 and -2 could be augmented in vivo by local transfection with the VEGF gene in the embryonic brain or by cutaneous wounding in adult mice. These results suggest that vasohibin-2, in combination with vasohibin-1, forms a novel family of angiogenesis inhibitors.

References

Jul 1, 1995·The Journal of Experimental Medicine·A L AngiolilloG Tosato
Feb 1, 1997·Endocrine Reviews·N Ferrara, T Davis-Smyth
Sep 9, 2000·Current Opinion in Cell Biology·J Lawler
May 1, 2001·Methods : a Companion to Methods in Enzymology·N Osumi, T Inoue
Feb 18, 2003·Arteriosclerosis, Thrombosis, and Vascular Biology·Tomowaki NakagawaYasufumi Sato
Jun 5, 2003·Nature Medicine·Peter Carmeliet
Jun 14, 2003·Circulation Research·Jean-Sébastien SilvestreBernard I Lévy
Mar 9, 2004·Biochemical and Biophysical Research Communications·Yasuhiro HasegawaYasufumi Sato
Jul 31, 2004·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Hyuek Jong LeeGou Young Koh

❮ Previous
Next ❯

Citations

Mar 23, 2012·Medical Oncology·Zhanlong ShenPauli Puolakkainen
Sep 5, 2013·International Journal of Hematology·Toru KawamotoYutaka Kohgo
Jan 11, 2011·Journal of Biochemistry·Yasufumi Sato
Oct 27, 2012·Journal of Biochemistry·Yasufumi Sato
Dec 3, 2010·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Thomas S LisseMartin Hewison
Mar 24, 2007·Nihon yakurigaku zasshi. Folia pharmacologica Japonica·Yasufumi Sato
Mar 1, 2012·Reproduction : the Official Journal of the Society for the Study of Fertility·Koumei ShirasunaAkio Miyamoto
May 27, 2006·Endothelium : Journal of Endothelial Cell Research·Yasufumi Sato
Nov 24, 2016·Protein Science : a Publication of the Protein Society·Tetsuya KadonosonoShinae Kizaka-Kondoh
Feb 5, 2010·Pharmaceuticals·Yasufumi Sato
Nov 20, 2016·Reproduction in Domestic Animals = Zuchthygiene·B BerishaM W Pfaffl
Sep 9, 2017·Cancer Biology & Therapy·Hua DuYonghong Shi
Feb 6, 2018·Journal of Atherosclerosis and Thrombosis·Yasufumi Sato
Jun 26, 2018·International Journal of Molecular Sciences·Katsuyuki TanabeJun Wada
Nov 11, 2006·Arteriosclerosis, Thrombosis, and Vascular Biology·Yasufumi Sato, Hikaru Sonoda
Mar 28, 2007·Journal of Cellular Physiology·Takahiro SuzukiYasufumi Sato
Mar 31, 2007·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Suresh NimmagaddaRuijin Huang
May 11, 2019·Cancer Science·Rie Iida-NoritaYasufumi Sato
Oct 19, 2017·Experimental and Therapeutic Medicine·Bo ZhangHailong Hu
Jan 26, 2020·Esophagus : Official Journal of the Japan Esophageal Society·Miho YamamotoYasufumi Sato
Jul 2, 2020·International Journal of Molecular Sciences·Hiromasa MiyakeJun Wada
Mar 6, 2015·Journal of Atherosclerosis and Thrombosis·Yasufumi Sato
Jul 25, 2012·Molecular Cancer Research : MCR·Yoshifumi TakahashiYasufumi Sato
Jun 8, 2012·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Yasumasa MiyazakiMototsugu Oya
Nov 8, 2020·International Journal of Molecular Sciences·Yujiro Kida

❮ Previous
Next ❯

Related Concepts

Related Feeds

Arterial-Venous in Development & Disease

Arterial-venous development may play a crucial role in cardiovascular diseases. Here is the latest research.