Isolation and characterization of Wharton's jelly-derived multipotent mesenchymal stromal cells obtained from bovine umbilical cord and maintained in a defined serum-free three-dimensional system.

BMC Biotechnology
Tereza C CardosoRoberto Gameiro

Abstract

The possibility for isolating bovine mesenchymal multipotent cells (MSCs) from fetal adnexa is an interesting prospect because of the potential for these cells to be used for biotechnological applications. Bone marrow and adipose tissue are the most common sources of MSCs derived from adult animals. However, little knowledge exists about the characteristics of these progenitors cells in the bovine species. Traditionally most cell cultures are developed in two dimensional (2D) environments. In mammalian tissue, cells connect not only to each other, but also support structures called the extracellular matrix (ECM). The three-dimensional (3D) cultures may play a potential role in cell biotechnology, especially in tissue therapy. In this study, bovine-derived umbilical cord Wharton's jelly (UC-WJ) cells were isolated, characterized and maintained under 3D-free serum condition as an alternative of stem cell source for future cell banking. Bovine-derived UC-WJ cells, collected individually from 5 different umbilical cords sources, were successfully cultured under serum-free conditions and were capable to support 60 consecutive passages using commercial Stemline(®) mesenchymal stem cells expansion medium. Moreover, the UC-WJ cells wer...Continue Reading

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Citations

Sep 18, 2013·Applied Biochemistry and Biotechnology·Taofeng LuWeijun Guan
Mar 8, 2014·Veterinary Research Communications·M D PratheeshG Taru Sharma
Jul 16, 2015·Expert Opinion on Biological Therapy·Michail E KlontzasAthanasios Mantalaris
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Dec 6, 2018·Journal of Cellular Physiology·Mudasir Bashir GugjooGutulla Taru Sharma
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Apr 20, 2021·Frontiers in Cell and Developmental Biology·Rebecca M HarmanGerlinde R Van de Walle
May 18, 2021·Frontiers in Veterinary Science·Lynn PezzaniteLaurie Goodrich

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Methods Mentioned

BETA
dissection
cesarean section
PMA

Software Mentioned

AxioVision®
AlgiMatrix®
SAS

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