May 21, 2016

Isolation and characterization of electrochemically active subsurface Delftia and Azonexus species

BioRxiv : the Preprint Server for Biology
Yamini JangirMohamed Y El-Naggar

Abstract

Continental subsurface environments can present significant energetic challenges to the resident microorganisms. While these environments are geologically diverse, potentially allowing energy harvesting by microorganisms that catalyze redox reactions, many of the abundant electron donors and acceptors are insoluble and therefore not directly bioavailable. Extracellular electron transfer (EET) is a metabolic strategy that microorganisms can deploy to meet the challenges of interacting with redox-active surfaces. Though mechanistically characterized in a few metal-reducing bacteria, the role, extent, and diversity of EET in subsurface ecosystems remains unclear. Since this process can be mimicked on electrode surfaces, it opens the door to electrochemical techniques to enrich for and quantify the activities of environmental microorganisms in situ. Here, we report the electrochemical enrichment of microorganisms from a deep fractured-rock aquifer in Death Valley, California, USA. In experiments performed in mesocosms containing a synthetic medium based on aquifer chemistry, four working electrodes were poised at different redox potentials (272, 373, 472, 572 mV vs. SHE) to serve as electron acceptors, resulting in anodic currents ...Continue Reading

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Mentioned in this Paper

Microorganism
Metabolic Process, Cellular
Azonexus
Electrochemical Techniques
Extracellular
Delftia
Oxidation-Reduction Activity [MoA]
Isolation Aspects
Environment
Electron Transport

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